食源性单增李斯特菌LPXTG蛋白基因的检测

发布时间：2018-06-04 00:24

本文选题：单核细胞增多性李斯特菌 + LPXTG基序表面蛋白　；参考：《中国兽医杂志》2017年08期

【摘要】：单核细胞增多性李斯特菌(Listeria monocytogenes,LM)作为四大重要食源性病原菌之一,对人类及多种畜禽健康威胁极大。LM细胞胞壁表面分布多种毒力蛋白,在感染机体过程中发挥重要作用,其中有一类至关重要的表面蛋白其C末端含有保守基序LPXTG,分选酶A能够识别基序LPXTG,共价结合到肽聚糖上,呈现在细胞表面发挥毒力作用。本试验以不同食品中分离得到的24株单增李斯特菌为试验对象,设计合成了41对预测含LPXTG基序表面蛋白的引物,利用PCR技术进行扩增,凝胶电泳成像系统检测扩增产物片段大小,计算24株食品源单增李斯特菌LPXTG基序表面蛋白的携带。结果表明,不同LM分离株LPXTG蛋白基因的携带率不同:其中仅有12.5%(3/24)的分离株携带率在85%以上;不同LPXTG蛋白基因的检出率不同,在41个检测对象中,有8个基因的检出率100%,有3个基因的检出率不足20%,Lmo1115基因在所有分离株中均未检测到。本研究对了解食品源LM分离株的LPXTG蛋白基因的分布以及探明食品源LM的致病性具有重要意义。
[Abstract]:Listeria monocytogenes (LM), one of the four important foodborne pathogens, is one of the four most important foodborne pathogens, which threatens the health of human and a variety of livestock and poultry with a variety of virulence proteins on the cell wall surface of.LM cells, and plays an important role in the process of infection. There is a class of critical surface proteins which contain the C end of its C. Based on the sequence LPXTG, the sorting enzyme A can identify the motif LPXTG and covalently bind to the peptidoglycan and present the toxic effect on the cell surface. In this experiment, 24 strains of Lester bacteria isolated from different foods were used as the test object, and 41 pairs of primers for predicting the surface protein containing LPXTG were designed and synthesized, and the PCR technique was used to amplify and gel electrophoresis. The size of the amplified product fragment was measured by the imaging system, and the carrying rate of the LPXTG base sequence surface protein of 24 strains of Lester bacteria was calculated. The results showed that the carrying rate of LPXTG protein gene in different LM isolates was different: only 12.5% (3/24) was more than 85%, and the detection rate of different LPXTG protein genes was different in 41 detection objects. The detection rate of 8 genes is 100%, the detection rate of 3 genes is less than 20%, and the Lmo1115 gene is not detected in all the isolates. This study is of great significance to understand the distribution of LPXTG protein gene in food source LM isolates and to explore the pathogenicity of food source LM.
【作者单位】：石河子大学动物科技学院;
【基金】：国家自然科学基金(31360614)
【分类号】：R155

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