顺铂染毒大鼠睾丸定量组织学分析及睾丸酶活力的变化

发布时间：2018-06-19 21:59

  本文选题：顺铂 + 定量组织学分析　； 参考：《毒理学杂志》2015年03期

【摘要】：目的 探讨顺铂(CP)染毒大鼠睾丸组织病理学和睾丸酶活力的变化。方法 选择健康成年雄性Wistar大鼠,随机分为CP 1.0、2.5和5.0 mg/kg组及对照组,连续腹腔注射染毒3 d。染毒结束后,制备PAS(Periodic AcidSchiff)染色病理切片,观察睾丸组织病理变化并行定量组织学分析。分光光度法测定琥珀酸脱氢酶(SDH)、苹果酸脱氢酶(MDH)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、诱导型一氧化氮合酶(i NOS)和Na+-K+-ATPase活力。实时荧光定量PCR检测i NOS和Na+-K+-ATPasemRNA表达水平。结果 CP染毒后大鼠睾丸组织病理学改变主要见于睾丸生精上皮精子发生周期第Ⅱ-Ⅲ期。定量组织学分析发现,在生精上皮第Ⅱ-Ⅲ期CP 2.5和5.0 mg/kg组大鼠睾丸生精小管相对面积和直径变小,管腔相对面积增加(P0.05)。CP各剂量组生精上皮占生精小管面积百分比和生精小管管腔直径均变小(P0.05),CP5.0 mg/kg组睾丸间质血管面积增加(P0.01)。与对照组比较,CP 5.0 mg/kg组精子数减少,精子活力降低(P0.05)。CP各染毒组SDH活力均低于对照组,而MDH和i NOS活力仅5.0 mg/kg组低于对照组(P0.05)。CP 5.0 mg/kg组ACP、AKP和Na+-K+-ATPase活力均明显高于对照组(P0.05)。RT-PCR结果显示,Na+-K+-ATPase和i NOS mRNA表达水平与酶活力改变一致。结论 顺铂可能参与改变睾丸细胞能量代谢相关酶活力而致睾丸细胞损伤。
[Abstract]:Objective to investigate the changes of testicular histopathology and testicular enzyme activity in rats exposed to cisplatin (CPD). Methods healthy adult male Wistar rats were randomly divided into 2. 5 and 5. 5 mg/kg groups of CP 1.0 and 5. 0 mg/kg and control group. The rats were injected intraperitoneally for 3 days. The pathological sections of PASA Periodic AcidSchiff staining were prepared and the pathological changes of testis were observed and the quantitative histological analysis was performed. The activities of succinate dehydrogenase (SDH), malate dehydrogenase (MDH), acid phosphatase (ACPP), alkaline phosphatase (ALP), inducible nitric oxide synthase (iNOS) and Na K ATPase were determined by spectrophotometry. The expression of iNOS and Na-K-ATPase mRNA were detected by real-time fluorescence quantitative PCR. Results the histopathological changes of testis were mainly observed in the spermatogenesis stage 鈪，

本文编号：2041461