1,2-二氯乙烷对SD大鼠亚急性吸入毒性研究

发布时间：2018-06-21 12:16

本文选题：-二氯乙烷 + 亚急性吸入　；参考：《中国职业医学》2015年04期

【摘要】：目的确定1,2-二氯乙烷(1,2-DCE)亚急性吸入的毒性作用和靶器官。方法无特定病原体级健康成年SD大鼠64只,雌雄各半,随机分为对照组、低剂量组和高剂量组。采用口鼻式动式吸入染毒法,低和高剂量组予质量浓度为600和1 800 mg/m3的1,2-DCE染毒8 h/d,连续7 d;对照组吸入新鲜空气。染毒期间称量大鼠体质量,观察其活动状况。实验结束后,检测大鼠血常规和血清血生化指标;解剖、分离主要脏器计算脏器系数,并进行组织病理学检查。结果染毒后第4~7天,高剂量组雌性和雄性大鼠体质量均分别低于同性别对照组(P0.05),并从染毒后第3天开始出现不同程度的体质量负增长(P0.05)。雌性高剂量组大鼠血清天冬氨酸氨基转移酶、γ-谷氨酰基转移酶、乳酸脱氢酶的活力和甘油三酯、尿素氮、肌酐的水平均高于雌性对照组(P0.05)。高剂量组大鼠脑、肝脏、肺脏的脏器系数均分别高于对照组(P0.05);与雌性对照组比较,雌性高剂量组大鼠心脏、肾脏和肾上腺的脏器系数均升高(P0.05),脾脏脏器系数下降(P0.05);雄性高剂量组大鼠肾脏和肾上腺的脏器系数均高于雄性对照组(P0.05)。肉眼可见大鼠脑、肝脏和肾脏大体形态学改变。组织病理学检查结果显示,高剂量组部分大鼠出现大脑皮质和血管周围空泡化,小脑颗粒细胞坏死等脑水肿病变;部分大鼠肝脏可见肝细胞水肿;部分大鼠肾脏可见肾小管蛋白沉积、肾小管上皮细胞水肿、肾小管扩张等病理改变,并可见肾小管上皮细胞异型性。各组大鼠血常规指标未出现有生物学意义的改变;低剂量组大鼠血清血生化指标基本未出现有生物学意义的改变。结论 1,2-DCE亚急性吸入可引起大鼠脑水肿、肝脏和肾脏损害;大脑、小脑、肝脏和肾脏是1,2-DCE亚急性吸入毒性作用的靶器官;1,2-DCE毒作用存在性别差异。
[Abstract]:Objective to determine the subacute inhalation toxicity and target organs of 1 chloride-2-dichloroethane. Methods Sixty-four healthy adult SD rats, half male and half female, were randomly divided into control group, low dose group and high dose group. The oral and nasal dynamic inhalation was used. The low and high dose groups were given 8 h / d of 1: 2-DCE at 600 and 1 800 mg/m3 for 7 consecutive days, while the control group inhaled fresh air for 7 days. The body mass of rats was measured during exposure and their activities were observed. After the experiment, the blood routine and serum biochemical indexes of rats were detected, and the main organs were dissected to calculate the organ coefficients, and histopathological examination was carried out. Results the body mass of female and male rats in the high dose group was lower than that in the same sex control group on the 7th day after exposure, and the body weight of the female and male rats increased negatively from the third day after exposure. The serum levels of aspartate aminotransferase, 纬 -glutamyl transferase, lactate dehydrogenase, triglyceride, urea nitrogen and creatinine in female high dose group were higher than those in female control group (P 0.05). The organ coefficients of brain, liver and lung in the high dose group were higher than those in the control group (P 0.05), and compared with the female control group, the heart of the rats in the high dose group was higher than that in the control group. The viscera coefficients of kidney and adrenal gland were all increased, the spleen organ coefficient was decreased, and the organ coefficients of kidney and adrenal gland of male high dose group were higher than that of male control group (P0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%). Gross morphological changes of brain, liver and kidney were observed in the naked eye. Histopathological examination showed that some rats in high dose group had brain edema, such as cerebral cortex and perivascular vacuolation, cerebellar granulosa cell necrosis and hepatocyte edema. The renal tubule protein deposition, renal tubular epithelial cell edema, renal tubule dilatation and other pathological changes were observed in some of the kidneys of the rats. No biological changes were found in the blood routine indexes of the rats in each group, and no biological changes were found in the serum biochemical indexes of the rats in the low dose group. Conclusion 1the subacute inhalation of 2-DCE can cause brain edema, liver and kidney damage in rats, and the target organ of 1h 2-DCE subacute inhalation toxicity is brain, cerebellum, liver and kidney.
【作者单位】：广东省职业病防治院广东省职业病防治重点实验室;中山大学公共卫生学院;山西医科大学公共卫生学院;
【基金】：国家自然科学基金青年项目(81202245) 广东省职业病防治重点实验室(2012A061400007) 广东省自然科学基金(S2013010011816) 广东省化学中毒与核辐射突发事件医学救援应急技术研究中心(2012A032100002) 广东省医学科学技术研究基金(B2014017,A2012068)
【分类号】：R135.1

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