2016年上海市松江区婴幼儿食品中阪崎肠杆菌污染状况分析

发布时间：2018-06-26 03:33

本文选题：阪崎肠杆菌 + 婴幼儿　；参考：《环境与职业医学》2017年07期

【摘要】：[目的]了解上海市松江区婴幼儿食品中阪崎肠杆菌的污染状况、分子分型及耐药性,为婴幼儿食品安全管理和食源性疾病的预警及临床用药提供科学依据。[方法]2016年选择松江区3个街道和4个乡镇,共19家食品店或超市,采集婴幼儿配方奶粉(包括乳基、豆基)、婴幼儿谷类辅助食品和适用于婴幼儿的固体饮料,共124件。依据GB 4789.40—2010《食品安全国家标准食品微生物学检验阪崎肠杆菌检验》检验程序及荧光定量PCR方法,对所采集样品进行阪崎肠杆菌的分离培养鉴定及血清分型,并进一步进行药物敏感性试验;采用脉冲场凝胶电泳(PFGE)技术进行分子分型。[结果]124件样品中:婴幼儿谷类辅食51件,检出阪崎肠杆菌11株(9株保存成功),阳性率为21.6%;婴幼儿配方奶粉53件、固体饮料20件,均未检出阪崎肠杆菌;3类食品中阪崎肠杆菌检出率的差异有统计学意义(P0.01)。采用PCR方法验证阳性菌,符合率为100%。9株阳性菌血清分型显示3株O1群,6株O2群。药敏试验结果显示,在所测定的16种抗生素中,9株阪崎肠杆菌只对头孢唑啉中度耐药或耐药,对其他抗生素均敏感。PFGE分子分型结果显示9株阪崎肠杆菌分为5种PFGE型别,相似度范围在50%~100%;其中A型有4株,B型有2株,相似度均为100%。[结论]上海市松江区婴幼儿谷类辅食中阪崎肠杆菌的阳性率高于其他类婴幼儿食品。阪崎肠杆菌对测定的抗生素(除头孢唑啉外)均敏感,其血清分型主要为O1、O2群,PFGE分型显示不同或相同品牌婴幼儿辅食中阪崎肠杆菌有同源性,可应用于阪崎肠杆菌的溯源。
[Abstract]:[objective] to investigate the contamination, molecular typing and drug resistance of Enterobacter sakazakii in infant food in Songjiang District, Shanghai, and to provide scientific basis for food safety management, early warning of foodborne diseases and clinical use of drugs. [methods] in 2016, 19 food stores or supermarkets were selected from 3 streets and 4 townships in Songjiang District, and 124 pieces of infant formula (including milk base, soybean base), supplementary food for infants and cereals and solid drinks for infants and young children were collected. According to the test procedure of Enterobacter sakazakii in food microbiology of the National Food Safety Standard and the fluorescent quantitative PCR method, the isolated, cultured and serotyping of Enterobacter sakazakii were carried out. The drug sensitivity test was further carried out and the molecular typing was carried out by pulsed field gel electrophoresis (PFGE). [results] among 124 samples, 51 samples of infant cereals were found, 11 strains of Enterobacter sakazakii (9 strains were successfully preserved) were detected, the positive rate was 21.60.53 pieces of infant formula milk powder and 20 pieces of solid drinks were detected. There was significant difference in the detection rate of Enterobacter sakazakii in three kinds of food (P0.01). PCR method was used to verify the positive bacteria, the coincidence rate was 100.9 positive bacteria serotyping showed 3 strains of O1 group and 6 strains of O2 group. The results of drug sensitivity test showed that 9 strains of Enterobacter sakazakii were only moderately resistant or resistant to cefazolin, and were sensitive to other antibiotics. The results of PFGE molecular typing showed that 9 strains of Enterobacter sakazakii were classified into 5 PFGE types. The similarity ranges from 50 to 100, in which there are 4 strains of type A and 2 strains of type B, and the similarity is 100%. [conclusion] the positive rate of Enterobacter sakazakii in infant cereals in Songjiang District of Shanghai is higher than that in other kinds of infant foods. Enterobacter sakazakii was sensitive to antibiotics (except cefazolin). The serotyping of Enterobacter sakazakii showed that there was homology of Enterobacter sakazakii in different or same brands of infantile supplementary food, which could be used to trace the origin of Enterobacter osakazakii.
【作者单位】：上海市松江区疾病预防控制中心微生物实验室;
【基金】：上海市松江区公共卫生合作项目-上海市松江地区腹泻病病原监测研究(编号:2014-12)
【分类号】：R155.5

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