烹调油烟对女（雌）性的性腺毒性作用

发布时间：2018-07-02 13:30

本文选题：烹调油烟 + 脏器系数　；参考：《福建医科大学》2012年硕士论文

【摘要】：目的本研究从流行病学现场调查和亚慢性动物实验两方面研究烹调油烟对女（雌）性的性腺毒性作用。方法 1、流行病学调查采用生殖流行病学调查方法，按照统一的妇女生殖健康调查表，整群抽样的方法抽取某社区女性厨师152名为暴露组，选择该社区超市（在家没有煮饭的女性）608名作为对照组。 2、亚慢性动物实验选用清洁级Wistar雌性大鼠50只，按体重随机分为5组，每组10只，，全身暴露烹调油烟，连续56天，按每天暴露时间分为低剂量组（0.5小时/天）、中剂量组（1小时/天）、高剂量组（2小时/天）、极高剂量组（4小时/天）及空白对照组（不做任何处理），第43天开始观察大鼠动情周期的变化。暴露结束后待大鼠处于动情期时取血清、卵巢和子宫，检测卵巢湿重及脏器系数、子宫湿重及脏器系数、各级卵泡数目构成比和卵巢细胞超微结构、性激素等。结果 1、流行病学调查烹调油烟暴露组女工经量异常、妊娠高血压、早产、自然流产、先天畸形等的检出率高于对照组(P0.05)；而腹痛、嗜睡失眠、恶心、妊娠贫血、妊娠剧吐、过期产等检出率两组间差异无统计学意义(P0.05)。 2、亚慢性动物实验各剂量组染毒后体重差异无统计学意义，体重增加值高于对照组（p0.05）；各剂量组卵巢湿重和脏器系数与对照组比较无统计学意义；各剂量组子宫湿重和脏器系数与对照组比较无统计学意义；极高剂量组的动情后期时间长于对照组（P0.05）；极高剂量组的原始卵泡的数目构成比显著低于对照组（p0.01）,低剂量组的闭锁卵泡的数目构成比显著高于对照组（p0.05），极高剂量组的黄、白体数目构成比显著高于对照组（p0.01）。透射电镜观察各剂量组卵巢生殖细胞超微结构发生改变。低剂量组LH水平显著下降而极高剂量组LH水平显著上升，差异有统计学意义（P0.01)；低剂量组P4水平显著上升，差异有统计学意义（P0.01）；低、中剂量组E2水平显著显著上升而极高剂量组E2水平显著下降，差异有统计学意义（P0.05）。结论烹调油烟暴露可使女性月经先兆状况、月经经量异常、妊娠高血压、早产、自然流产、先天畸形的发生率增加。在本实验条件下，烹调油烟使动情后期时间延长，各级卵泡的数目构成比和卵巢生殖细胞超微结构发生改变，性激素水平改变。因此，烹调油烟对女（雌）性的性腺有一定的毒性作用。
[Abstract]:Objective to study the gonadal toxicity of cooking oil fume on female (female) from two aspects of epidemiological investigation and subchronic animal experiment. Methods 1. The method of reproductive epidemiology survey was used to select 152 female cooks from a community as exposure group according to the unified questionnaire of women's reproductive health and cluster sampling. 608 women from the community supermarket (women without cooking at home) were selected as the control group. 2. Fifty female Wistar rats of clean grade were selected in the subchronic animal experiment. They were randomly divided into 5 groups according to their body weight, 10 rats in each group. Whole body exposure cooking oil fumes, 56 days in a row, According to daily exposure time, they were divided into low dose group (0.5 hours / day), middle dose group (1 hour / day), high dose group (2 hours / day), very high dose group (4 hours / day) and blank control group (no treatment). To observe the change of estrus cycle in rats. Serum, ovary and uterus were taken from rats in estrous period after exposure. The wet weight of ovary and organ coefficient, uterine wet weight and organ coefficient, ratio of follicle number of different grades, ultrastructure of ovarian cell, sex hormone and so on were detected. Results 1. The prevalence of abnormal quantity, pregnancy hypertension, premature delivery, spontaneous abortion and congenital malformation of female workers in cooking oil fume exposure group was higher than that in control group (P0.05), but abdominal pain, sleepiness, insomnia, nausea, etc. There was no significant difference between the two groups in the detection rate of anemia, hyperemesis, and late delivery (P0.05). 2. There was no significant difference in body weight after exposure to each dose of subchronic animal experiment, and the weight gain was higher than that in the control group (p0.05). The ovarian wet weight and organ coefficient of each dose group had no statistical significance compared with the control group; the uterine wet weight and organ coefficient of each dose group had no statistical significance compared with the control group; the estrus late period of the extremely high dose group was longer than that of the control group (P0.05). The ratio of the number of primary follicles in the very high dose group was significantly lower than that in the control group (p0.01), the ratio of the number of atretic follicles in the low dose group was significantly higher than that in the control group (p0.05), and the ratio of the number of yellow and white bodies in the extremely high dose group was significantly higher than that in the control group (p0.01). The ultrastructure of ovarian germ cells in each dose group was observed by transmission electron microscope. The LH level in the low dose group decreased significantly while that in the very high dose group increased significantly (P0.01), while the P4 level in the low dose group increased significantly (P0.01), the difference was significant in the low dose group (P0.01), the difference was significant in the low dose group (P0.01). The level of E 2 in middle dose group increased significantly while that in very high dose group decreased significantly (P0.05). Conclusion Cooking oil smoke exposure can increase the incidence of menstrual precursor, menstrual volume abnormality, pregnancy hypertension, premature delivery, spontaneous abortion and congenital malformation. In this experiment, cooking oil fume prolonged the estrus, the ratio of the number of follicles, the ultrastructure of ovarian germ cells, and the level of sex hormone. Therefore, cooking oil fumes have a certain toxic effect on female (female) gonads.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R114

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