对氨基水杨酸钠对锰致大鼠肝抗氧化酶和病理学改变的影响

发布时间：2018-07-17 18:52

【摘要】：目的探讨对氨基水杨酸钠(PAS-Na)对锰致大鼠肝微粒体、线粒体抗氧化酶及病理学改变的影响。方法50只雄性SD大鼠按体重随机分为对照组和染锰组。染锰组腹腔注射(ip)Mn Cl2·4H2O 15 mg/kg,对照组ip等容量生理盐水,1次/d,每周5 d,连续6周。随后,将染锰组按体重随机分为染锰、低、中、高剂量PAS-Na治疗(L、M、H-PAS)组。L、M、H-PAS组大鼠分别背部皮下注射(sc)PAS-Na 100、200和300 mg/kg,对照组、染锰组背部sc等容量生理盐水,1次/d,每周4 d,连续4周。测定肝线粒体、微粒体超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,并观察肝组织病理学改变。结果与对照组比较,染锰组肝线粒体SOD和GSH-Px、微粒体SOD活性降低,但差异无统计学意义(P0.05)。染锰组肝脏系数增大,肝脏汇管区出现炎性淋巴细胞及中性粒细胞浸润,肝小叶内出现点状坏死,甚至部分灶性坏死,部分肝细胞出现脂肪变性;与染锰组比较,M-PAS组点状坏死灶明显减少,L、H-PAS组点状坏死灶也有所减少。结论染锰可使大鼠肝脏汇管区出现炎性淋巴细胞及中性粒细胞浸润,肝小叶点状坏死,甚至部分灶性坏死,肝细胞脂肪变性,PAS-Na治疗可使其病理形态学改变明显好转。
[Abstract]:Objective to investigate the effects of sodium p-aminosalicylate (PAS-Na) on hepatic microsomes, mitochondrial antioxidant enzymes and pathological changes in rats induced by manganese. Methods 50 male Sprague-Dawley rats were randomly divided into two groups according to their body weight: control group and manganese group. In the manganese exposed group, (ip) mn Cl 2 4H 2O was injected intraperitoneally at 15 mg / kg, while in the control group, the same volume of normal saline was given once a day, 5 days a week for 6 weeks. Then, according to the body weight, the rats in the manganese exposed group were randomly divided into three groups: manganese, low, medium and high dose PAS-Na treatment group. The rats were subcutaneously injected with (sc) PAS-Na 100200 and 300mg / kg in the back of the control group and the manganese exposed group respectively. The rats in the control group and the manganese exposed group received sc isovolumic physiological saline once a day, 4 days a week for 4 weeks. The activities of liver mitochondria, microsomal superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured. Results compared with the control group, the activities of SOD and GSH-Px in liver mitochondria and microsomal SOD in manganese exposed group were lower than those in control group, but there was no significant difference (P0.05). In the manganese exposed group, the liver coefficient increased, inflammatory lymphocytes and neutrophils infiltrated in the hepatic catchment area, dotted necrosis or even partial focal necrosis in the hepatic lobules, and fatty degeneration in some liver cells. Compared with manganese group, the point necrotic foci in M-PAS group were significantly reduced, and that in LH-PAS group was also decreased. Conclusion Manganese can make inflammatory lymphocytes and neutrophils infiltrate in the hepatic catchment area of rats, and hepatic lobular dotted necrosis, even partial focal necrosis. PAS-Na treatment of hepatocyte steatosis can obviously improve the pathomorphologic changes of rats.
【作者单位】：广西医科大学公共卫生学院卫生毒理学教研室;广西医科大学第一附属医院病理科;
【基金】：国家自然科学基金(81072320,81460505) 广西自然科学基金(2014GXNSFAA118232)
【分类号】：R114

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