蛋白质二硫键异构酶在染砷家兔皮肤中的表达变化
发布时间：2018-08-14 14:10
【摘要】：目的观察亚砷酸钠(i As3+)染毒对家兔皮肤蛋白质二硫键异构酶(protein disulfide isomerase,PDI)的影响,探讨PDI与砷致皮肤损害的关系。方法将30只健康雄性家兔随机平均分到对照组(C)及低(L,0.13mg/千克体重(kg.w))、中(M,0.26mg/kg.w)、中高(MH,0.65mg/kg.w)、高(H,1.30mg/kg.w)剂量染毒组。亚砷酸钠饮水染毒3个月后处死,取背部相同位置全厚皮肤样。双向凝胶电泳分离样品中蛋白,Bio-Rad图像系统分析胶图,筛选差异表达蛋白,胶内酶切后采用基质辅助激光解析电离飞行时间质谱进行鉴定。通过实时荧光定量聚合酶链反应(real-time polymerase chain reaction,RT-PCR)检测样品中PDI mRNA的表达。结果染毒3个月后,家兔无死亡,各组家兔体重无明显变化,M、MH、H组可观察到家兔背腹部毛发无光泽、粗糙,H组尤为明显。皮肤中PDI在M、MH组表达量下降,在H组表达量上升。PDI mRNA在MH和H组上调(F=225.83,P0.001)。结论亚砷酸钠染毒后,随染毒剂量升高,家兔皮肤PDI蛋白表达量先降低后升高,PDI mRNA逐渐增高,同时可观察到家兔毛发粗糙,提示PDI可能在砷致皮肤损伤中发挥作用。
[Abstract]:Objective to observe the effect of sodium arsenite (I As3) on (protein disulfide isomeraseisomerase (protein disulfide isomerase) in rabbit skin, and to explore the relationship between PDI and arsenic induced skin damage. Methods 30 healthy male rabbits were randomly divided into three groups: control group (C) 0.13 mg / kg), low (kg.w), 0.26 mg / kg 路w), moderate high (MHN 0.65 mg / kg 路w) and high dose (1.30 mg / kg 路w) groups. Sodium arsenite was killed after drinking water for 3 months. Bio-Rad image system was used to isolate proteins from samples by two-dimensional gel electrophoresis. Differential expression proteins were screened and identified by matrix assisted laser desorption ionization time of flight mass spectrometry (TOF-MS). The expression of PDI mRNA was detected by real-time real-time polymerase chain reaction-polymerase chain reaction (RT-PCR). Results after 3 months of exposure, there was no death in rabbits, and there was no obvious change in body weight in each group. The expression of PDI in the skin decreased in MH group, and increased in H group. PDI mRNA was up-regulated in MH and H group (FF225.83, P0.001). Conclusion after sodium arsenite exposure, the expression of PDI protein in rabbit skin decreases first and then increases gradually with the increase of the dose of arsenite. It is also observed that the hair of rabbits is rough, suggesting that PDI may play a role in arsenic induced skin injury.
【作者单位】： 新疆医科大学公共卫生学院劳动卫生与环境卫生学教研室;
【基金】：国家自然科学基金(81560513;81260410) 教育部高等学校博士学科点基金(20126517110002;20126517120003)
【分类号】：R114
本文编号：2183106
[Abstract]:Objective to observe the effect of sodium arsenite (I As3) on (protein disulfide isomeraseisomerase (protein disulfide isomerase) in rabbit skin, and to explore the relationship between PDI and arsenic induced skin damage. Methods 30 healthy male rabbits were randomly divided into three groups: control group (C) 0.13 mg / kg), low (kg.w), 0.26 mg / kg 路w), moderate high (MHN 0.65 mg / kg 路w) and high dose (1.30 mg / kg 路w) groups. Sodium arsenite was killed after drinking water for 3 months. Bio-Rad image system was used to isolate proteins from samples by two-dimensional gel electrophoresis. Differential expression proteins were screened and identified by matrix assisted laser desorption ionization time of flight mass spectrometry (TOF-MS). The expression of PDI mRNA was detected by real-time real-time polymerase chain reaction-polymerase chain reaction (RT-PCR). Results after 3 months of exposure, there was no death in rabbits, and there was no obvious change in body weight in each group. The expression of PDI in the skin decreased in MH group, and increased in H group. PDI mRNA was up-regulated in MH and H group (FF225.83, P0.001). Conclusion after sodium arsenite exposure, the expression of PDI protein in rabbit skin decreases first and then increases gradually with the increase of the dose of arsenite. It is also observed that the hair of rabbits is rough, suggesting that PDI may play a role in arsenic induced skin injury.
【作者单位】： 新疆医科大学公共卫生学院劳动卫生与环境卫生学教研室;
【基金】：国家自然科学基金(81560513;81260410) 教育部高等学校博士学科点基金(20126517110002;20126517120003)
【分类号】：R114
【相似文献】
相关期刊论文 前5条
1 高琦,文春河,王喜爱;铜尘对家兔皮肤损害的实验研究[J];卫生毒理学杂志;1992年02期
2 陈国建;邹凡凯;石萌萌;任晓慧;张浩;;紫外线A对家兔皮肤的氧化损伤研究[J];现代预防医学;2014年03期
3 王鸿;高俊宏;岳红;刘志永;李江平;张志忠;周彦水;;3,4-双(4’-氨基呋咱基-3’)氧化呋咱对家兔皮肤刺激性研究[J];环境与健康杂志;2013年06期
4 邬伯安;孙国章;张敏;;碘聚乙二醇复合物毒性观察[J];中国消毒学杂志;1990年01期
5 ;[J];;年期
，本文编号：2183106
本文链接：https://www.wllwen.com/yixuelunwen/yufangyixuelunwen/2183106.html
