杂粮早餐粉对砷致小鼠生殖毒性的缓解作用

发布时间：2018-08-23 20:42

【摘要】：以昆明种纯系雄性小白鼠为受试动物,检测杂粮早餐粉对砷(As)引发的生殖毒性的影响。As染毒组小鼠饮用水中含10mg/L的As,干预组在As染毒的同时用杂粮早餐粉灌胃,6周后检测小鼠睾丸组织的抗氧化指标,并对睾丸组织结构进行分析,统计精子畸形率。结果表明:通过饮用水摄入As能诱发实验动物睾丸组织氧化胁迫,使超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性下降,还原型谷胱甘肽(GSH)含量下降,脂质过氧化产物丙二醛(MDA)含量显著(P0.05)升高,睾丸组织结构出现病理性改变,精子异常率显著(P0.05)升高;用杂粮早餐粉干预后,小鼠睾丸组织的SOD和CAT活性提高,GSH含量升高,MDA含量降低,睾丸组织结构趋于正常,精子畸形率下降。上述结果表明,所用杂粮早餐粉能使砷染毒动物的睾丸组织结构和功能趋于对照组,对促进男性健康具有积极的作用。
[Abstract]:Kunming pure male mice were used as subjects. The effects of breakfast meal on reproductive toxicity induced by arsenic (As). The As, intervention group with 10mg/L in drinking water of as group was treated with As at the same time. The antioxidant index of testis of mice was detected after 6 weeks of oral administration of breakfast meal. The structure of testis was analyzed and the rate of sperm malformation was counted. The results showed that drinking water intake of As could induce oxidative stress in testis, decrease the activities of superoxide dismutase (SOD) and catalase (CAT), and decrease the content of reduced glutathione (GSH). The malondialdehyde (MDA) content of lipid peroxidation product increased significantly (P0.05), the pathological changes of testis tissue structure and the abnormal rate of sperm (P0.05). The activities of SOD and CAT in testis of mice were increased. The content of SOD and CAT in testis increased and the content of MDA decreased, the structure of testis tended to be normal, and the rate of sperm malformation decreased. The results showed that the breakfast meal could make the structure and function of testis of arsenic-exposed animals tend to control group and play a positive role in promoting male health.
【作者单位】：山西大学生命科学学院;
【基金】：国家自然科学基金(30870454;30470318;31371868) 优秀人才科技创新项目(201605D211031)
【分类号】：R114

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