变性淀粉的抗消化性和两种抗性淀粉对高脂饮食小鼠抗氧化能力影响的研究
发布时间：2018-08-31 09:02
【摘要】：以大米淀粉,玉米淀粉,糯玉米淀粉,糯米淀粉,甘薯淀粉和马铃薯淀粉为原料,对其进行乙酰化、辛烯基琥珀酸酯化、羟丙基醚化和交联四种变性,对其部分理化性质和抗消化性进行比较研究。然后选用两种复合变性淀粉(HP-CL和CL-OSA),并对其对高脂膳食小鼠的抗氧化能力进行了研究,旨在为功能性淀粉应用提供依据。本文主要研究结果如下： 首先,分别对六种原淀粉进行化学变性并比较其部分的理化性质,结果表明：乙酰化,OSA酯化和羟丙基化都能增加淀粉峰值粘度,降低糊化温度,但是有些淀粉OSA酯化变性后峰值粘度最大,有些淀粉羟丙基化后峰值粘度最大,高交联由于高温下不糊化,不具有粘度：乙酰化,OSA酯化和羟丙基化都能增加淀粉的透明度,其中糯性淀粉以OSA酯化变性增幅最大,马铃薯以醋酸酯化变性增幅最大,其余淀粉以羟丙基化增加幅度最大,交联降低所有淀粉的透明度；乙酰化,OSA酯化和羟丙基化都能增加淀粉的抗凝沉性,其中不论哪种原料均以OSA酯化变性最优；淀粉的冻融稳定性经乙酰化,OSA酯化和羟丙基化后都会有所增加,对不同的淀粉其改良程度不同,其中以羟丙基变性最能改善淀粉的冻融稳定性。 其次,研究不同化学变性淀粉的抗消化性,分析表明：乙酰化,辛烯基琥珀酸酯化,羟丙基醚化和交联均能降低淀粉RDS含量,增加淀粉的RS含量,且在增加淀粉的RS含量方面为羟丙基醚化乙酰化交联辛烯基琥珀酸酯化,个别淀粉稍有差异,其中辛烯基琥珀酸酯化主要是提高淀粉的SDS含量。 最后,研究HP-CL和CL-OSA高脂饮食C57BL/6J小鼠抗氧化作用的影响。C57BL/6J小鼠60只,随机分为5组,包括对照组(CL)、高脂组(HF)、高脂饲料中添加甘薯原淀粉(HF-SPS)、羟丙基交联甘薯淀粉(HF-HPCL-SPS)、交联酯化甘薯淀粉(HF-CLOSA-SPS),小鼠进行自由采食,12周后测定小鼠血清和肝脏中总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)、过氧化氢酶(CAT)。试验结果表明：HF-HPCL-SP组与HF组比较,血清的T-AOC活性、GSH-Px活性显著提高(P0.05),SOD活性、CAT活性有升高趋势,MDA浓度有下降趋势；肝脏的T-AOC活性、SOD活性显著提高(P0.05), CAT活性有升高趋势,GSH-Px活性无明显变化,MDA浓度显著降低(P0.05)。HF-CLOSA-SPS与HF组比较,血清的T-AOC活性有升高趋势,MDA浓度有下降趋势；肝脏的T-AOC活性显著提高(P0.05),SOD活性有升高趋势,MDA浓度有下降趋势。综上考虑复合变性淀粉HP-CL和CL-OSA均能改善高脂饮食小鼠的体内氧化应激水平,HP-CL改善作用优于CL-OSA。
[Abstract]:Rice starch, corn starch, glutinous rice starch, sweet potato starch and potato starch were treated with acetylation, octenyl succinic acid esterification, hydroxypropyl etherification and crosslinking. Some physicochemical properties and anti-digestibility were compared. Then two kinds of compound modified starch (HP-CL and CL-OSA) were selected and their antioxidant ability to high-fat diet mice were studied in order to provide the basis for the application of functional starch. The main results are as follows: firstly, six kinds of starch were chemically denatured and some physicochemical properties were compared. The results showed that acetylated OSA esterification and hydroxypropylation could increase the peak viscosity of starch. The gelatinization temperature was decreased, but the peak viscosity of some starch was the highest after esterification of OSA, and the peak viscosity of some starch was the highest after hydroxypropylation. No viscosity: acetylated OSA esterification and hydroxypropylation can increase the transparency of starch. The waxy starch has the largest increase in esterification with OSA, the largest increase in the esterification of potato in acetate, and the largest increase in hydroxypropylation in the remaining starch. The cross-linking decreased the transparency of all starch, the esterification of acetylated OSA and hydroxypropylation increased the anticoagulability of starch, and no matter which raw material was modified by OSA, the esterification of starch was the best. The freeze-thaw stability of starch increased after acetylated OSA esterification and hydroxypropylation, and the degree of improvement of different starch was different, among which hydroxypropyl modification could improve the freeze-thaw stability of starch. The results showed that acetylation, octenyl succinate esterification, hydroxypropyl etherification and crosslinking could decrease the RDS content and increase the RS content of starch. The RS content of starch was increased by hydroxypropyl etherification and crosslinked octenyl succinic acid esterification, and there was a slight difference among some starch, among which octenyl succinic acid esterification mainly increased the SDS content of starch. Finally, 60 C57BL / 6J mice were randomly divided into 5 groups. The total antioxidant capacity (T-AOC) of serum and liver were measured after 12 weeks of free feeding in (CL), high fat group (control group), in which sweet potato raw starch (HF-SPS), hydroxypropyl cross-linked sweet potato starch (HF-HPCL-SPS) and cross-linked esterified sweet potato starch (HF-CLOSA-SPS) were added to (HF), high-fat diet of control group. Superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malondialdehyde (MDA), catalase (CAT). The results showed that the activity of T-AOC and GSH-Px in the serum of HF group increased significantly (P0.05) the activity of cat increased and the concentration of malondialdehyde (MDA) decreased in the group of HF-HPCL-SP compared with the group of HF. The activity of T-AOC in liver was significantly increased (P0.05). (P0.05) the activity of GSH-Px did not change significantly. The concentration of T-AOC decreased significantly (P0.05). Compared with the group of HF, the activity of T-AOC in serum increased and the concentration of T-AOC decreased. The activity of T-AOC in liver was significantly increased (P0.05). Considering that both compound modified starch HP-CL and CL-OSA can improve the level of oxidative stress in mice with high fat diet, HP-CL can improve the effect of HP-CL better than that of CL-OSA..
【学位授予单位】：四川农业大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：TS235;R151.2
本文编号：2214524
[Abstract]:Rice starch, corn starch, glutinous rice starch, sweet potato starch and potato starch were treated with acetylation, octenyl succinic acid esterification, hydroxypropyl etherification and crosslinking. Some physicochemical properties and anti-digestibility were compared. Then two kinds of compound modified starch (HP-CL and CL-OSA) were selected and their antioxidant ability to high-fat diet mice were studied in order to provide the basis for the application of functional starch. The main results are as follows: firstly, six kinds of starch were chemically denatured and some physicochemical properties were compared. The results showed that acetylated OSA esterification and hydroxypropylation could increase the peak viscosity of starch. The gelatinization temperature was decreased, but the peak viscosity of some starch was the highest after esterification of OSA, and the peak viscosity of some starch was the highest after hydroxypropylation. No viscosity: acetylated OSA esterification and hydroxypropylation can increase the transparency of starch. The waxy starch has the largest increase in esterification with OSA, the largest increase in the esterification of potato in acetate, and the largest increase in hydroxypropylation in the remaining starch. The cross-linking decreased the transparency of all starch, the esterification of acetylated OSA and hydroxypropylation increased the anticoagulability of starch, and no matter which raw material was modified by OSA, the esterification of starch was the best. The freeze-thaw stability of starch increased after acetylated OSA esterification and hydroxypropylation, and the degree of improvement of different starch was different, among which hydroxypropyl modification could improve the freeze-thaw stability of starch. The results showed that acetylation, octenyl succinate esterification, hydroxypropyl etherification and crosslinking could decrease the RDS content and increase the RS content of starch. The RS content of starch was increased by hydroxypropyl etherification and crosslinked octenyl succinic acid esterification, and there was a slight difference among some starch, among which octenyl succinic acid esterification mainly increased the SDS content of starch. Finally, 60 C57BL / 6J mice were randomly divided into 5 groups. The total antioxidant capacity (T-AOC) of serum and liver were measured after 12 weeks of free feeding in (CL), high fat group (control group), in which sweet potato raw starch (HF-SPS), hydroxypropyl cross-linked sweet potato starch (HF-HPCL-SPS) and cross-linked esterified sweet potato starch (HF-CLOSA-SPS) were added to (HF), high-fat diet of control group. Superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malondialdehyde (MDA), catalase (CAT). The results showed that the activity of T-AOC and GSH-Px in the serum of HF group increased significantly (P0.05) the activity of cat increased and the concentration of malondialdehyde (MDA) decreased in the group of HF-HPCL-SP compared with the group of HF. The activity of T-AOC in liver was significantly increased (P0.05). (P0.05) the activity of GSH-Px did not change significantly. The concentration of T-AOC decreased significantly (P0.05). Compared with the group of HF, the activity of T-AOC in serum increased and the concentration of T-AOC decreased. The activity of T-AOC in liver was significantly increased (P0.05). Considering that both compound modified starch HP-CL and CL-OSA can improve the level of oxidative stress in mice with high fat diet, HP-CL can improve the effect of HP-CL better than that of CL-OSA..
【学位授予单位】：四川农业大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：TS235;R151.2
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