碳酸二乙酯对大鼠的致畸敏感期毒性研究

发布时间：2018-09-02 07:25

【摘要】：目的观察碳酸二乙酯(Diethyl Carbonate,DEC)对大鼠胚胎及胎仔的发育毒性和致畸作用。方法将DEC按500、200和80 mg/kg于大鼠妊娠第6~15天连续10 d灌胃给药。观察孕鼠一般反应、体重、摄食量变化,于妊娠第20天解剖孕鼠,记录黄体数、着床数、吸收胎、死胎、活胎进行计数,对胎仔的体重、身长、尾长以及外观形态、胎盘质量等进行评价,各窝1/2胎仔作骨骼畸形检查,另1/2胎仔作内脏检查。结果 DEC高、中、低剂量组孕鼠均未出现任何明显母体毒副反应;对孕鼠的体重增长和摄食量无明显影响;对胚胎形成与胎鼠的生长发育未见明显影响;对胎鼠的外观形态、内脏发育未见明显影响;对高剂量组胎鼠顶骨、胸骨、骶尾椎骨,中剂量胎鼠胸骨、骶尾椎骨发育有一定的骨化不全或骨化迟缓影响,但未见明显的胎仔畸形,致畸指数3.14。结论在本试验条件下,DEC对SD大鼠母体一般状况和子代发育的未观察到明显不良反应剂量(NOEAL)为80 mg/kg。
[Abstract]:Objective to observe the developmental toxicity and teratogenicity of diethyl carbonate (Diethyl Carbonate,DEC) on rat embryo and fetus. Methods DEC was administered intragastrically at 500200 and 80 mg/kg on the 6th day of gestation for 10 days. The changes of general reaction, body weight and food intake of pregnant rats were observed. The pregnant rats were dissected on the 20th day of gestation, the number of corpus luteum, the number of implantation, the number of absorbed fetus, dead fetus and live fetus were recorded, and the body weight, body length, tail length and appearance of fetus were counted. The quality of placenta was evaluated, bone deformities were examined in 1 / 2 litter and visceral examination was performed in 1 / 2 foetus. Results in the high, medium and low dose groups of DEC, there was no obvious maternal toxicity, no significant effect on the weight gain and food intake of the pregnant mice, no obvious effect on the embryonic formation and the growth and development of the fetal mice, and no obvious effect on the appearance of the fetal mice. The visceral development had no obvious effect on the development of parietal bone, sternum, sacrocaudal vertebra, middle dose fetal rat sternum and sacrocaudal vertebra, but there was no obvious fetal malformation and teratogenicity index of 3.14. Conclusion under the condition of this experiment, the dose of adverse reaction (NOEAL) was 80 mg/kg. in the normal maternal condition and the development of offspring of SD rats.
【作者单位】：浙江省医学科学院安全性评价研究中心;
【基金】：浙江省中医药管理局项目(2014ZA016) 浙江省科学技术厅项目(2013F20005)
【分类号】：R114

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