神经毒性体外评价系统研究进展

发布时间：2018-09-02 10:13

【摘要】：神经毒性体外评价系统是以细胞培养、组织培养等实验方法为基础,利用生物化学、分子学、电生理学、形态学检验以及各种组学技术对毒物暴露致神经系统结构或功能损伤进行研究的评价体系。应用领域包括高通量筛选神经毒性化学药物和神经毒性作用机制的研究。体外评价系统与标准化的体内神经毒性实验相比,其优势在于灵敏度高、可重复性强,是更加有效和经济的替代方法,能够补充或增加体内实验数据。虽然体外评价系统具有诸多优点,但也不能完全替代更加复杂和完整的神经系统。如何将体外实验数据与体内实验数据相关联是研究者关注的主要领域。体外评价系统的研究不断发展,如细化检测终点、构建特定的组织培养模型以及拓展简化系统实现跨学科间的创新等。神经毒性体外评价系统不仅仅是风险性评价的主要方法,还是研究多类型神经毒物作用机制的重要方法。
[Abstract]:Neurotoxicity evaluation system in vitro is based on cell culture, tissue culture and other experimental methods, using biochemical, molecular, electrophysiology, An evaluation system for structural or functional damage to the nervous system caused by exposure to toxic substances by morphological examination and various techniques. Applications include high-throughput screening of neurotoxic chemicals and mechanisms of neurotoxicity. Compared with the standard in vivo neurotoxicity test, the in vitro evaluation system has the advantages of high sensitivity, strong repeatability, and is a more effective and economical alternative method, which can supplement or increase in vivo experimental data. Although in vitro evaluation system has many advantages, it can not completely replace the more complex and complete nervous system. How to correlate in vitro experimental data with in vivo experimental data is a major area of concern for researchers. The research of in vitro evaluation system has been developing continuously, such as refinement of detection endpoint, construction of specific tissue culture model and expansion of simplified system to realize interdisciplinary innovation. Neurotoxicity in vitro evaluation system is not only the main method of risk assessment, but also an important method to study the mechanism of neurotoxicity.
【作者单位】：中国食品药品检定研究院国家药物安全评价监测中心;
【基金】：国家“重大新药创制”科技重大专项(2012ZX09302001)
【分类号】：R114

【共引文献】