硒的血管调节作用及对巨噬细胞摄取低密度脂蛋白的影响
发布时间：2018-09-10 09:52
【摘要】：本文用HUEVC体外血管生成模型,考察了硒杂环化合物(邻菲Up琳硒二唑)对血管生成的影响,用体外酶活性动力模型评价了富硒螺旋藻多肽对血管紧张素转化酶(ACE)的抑制作用,用动物实验及原代细胞培养研究了硒对巨噬细胞吞噬脂质的影响。实验内容和主要结果如下： 1采用细胞划痕实验及体外血管生成技术,检测邻菲Up琳硒二唑对HUEVC体外血管生成的影响。发现邻菲Up琳硒二唑对HUEVC细胞的迁移有明显抑制作用,且具有剂量效应。进而发现,随邻菲Up琳硒二唑处理浓度的增加,在4-6μM时,血管样结构比空白对照组明显变短、不完整,在8μM SePhen作用下,管状结果崩解,细胞凋亡增加。 2严格硒营养控制复制小鼠低硒、适硒和高硒模型,分离诱导相应不同硒营养状况的骨髓来源巨噬细胞(BMDM),并分别以80、160、320ng/mL硒浓度进行BMDM培养,发现高硒营养水平对活化巨噬细胞硒酶活性、ROS生成有促进作用,并且发现用低密度脂蛋白(LDL)处理BMDM5h后,介导脂质内吞的主要受体CD36在随硒营养水平增加而明显下调,RT-PCR发现CD36mRNA水平随硒营养水平提高有一定程度的上调,但无统计显著性。另外,无论蛋白水平还是mRNA水平均未检测到硒营养对巨噬细胞脂质摄取相关分子PPARγ有明显影响。 综上所述,邻菲Up琳硒二唑在高浓度8μM下抑制细胞迁移和血管生成。亚硒酸钠在高浓度下(320ng/mL)可能主要通过氧化还原平衡调节CD36降解,使巨噬细胞内吞脂质减少,对动脉粥样硬化具有一定的防治意义。
[Abstract]:The effects of selenium heterocyclic compounds (o-phenanthroline selenediazole) on angiogenesis were investigated using HUEVC angiogenesis model in vitro. The inhibitory effects of selenium-enriched phycopeptides on angiotensin converting enzyme (ACE) were evaluated by using in vitro enzyme activity dynamic model. The effect of selenium on the phagocytosis of macrophages was studied by animal experiment and primary cell culture. The experimental contents and main results were as follows: 1 the effect of phenanthroline Up on HUEVC angiogenesis in vitro was detected by cell scratch test and in vitro angiogenesis technique. It was found that o-phenanthrene Up linoselenediazole inhibited the migration of HUEVC cells in a dose-dependent manner. It was further found that with the increase of the concentration of o-phenanthrene Up linoselenediazole, at 4-6 渭 M, the vasculoid structure became shorter and incomplete than that of the blank control group, and the tubular results disintegrated under the action of 8 渭 M SePhen. Cell apoptosis increased. 2 mice models of low selenium, suitable selenium and high selenium were established under strict selenium nutrition control. (BMDM), from bone marrow macrophages with different selenium status were isolated and cultured with 80160320ng/mL selenium concentration respectively. It was found that high selenium nutrition level promoted the production of Ros in activated macrophages, and that BMDM5h was treated with low density lipoprotein (LDL) (LDL). CD36, the main receptor of lipid endocytosis, was down-regulated by RT-PCR with the increase of selenium nutrition level. It was found that the level of CD36mRNA was up-regulated to some extent with the increase of selenium nutrition level, but there was no statistical significance. In addition, no significant effect of selenium on lipid uptake of PPAR 纬 in macrophages was found in either protein level or mRNA level. In conclusion, o-phenanthroline Up linselenediazole inhibited cell migration and angiogenesis at high concentration of 8 渭 M. Sodium selenite at high concentration (320ng/mL) may regulate the degradation of CD36 by redox equilibrium and decrease the endocytosis lipid of macrophages, which has a certain significance for the prevention and treatment of atherosclerosis.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R151
本文编号：2234103
[Abstract]:The effects of selenium heterocyclic compounds (o-phenanthroline selenediazole) on angiogenesis were investigated using HUEVC angiogenesis model in vitro. The inhibitory effects of selenium-enriched phycopeptides on angiotensin converting enzyme (ACE) were evaluated by using in vitro enzyme activity dynamic model. The effect of selenium on the phagocytosis of macrophages was studied by animal experiment and primary cell culture. The experimental contents and main results were as follows: 1 the effect of phenanthroline Up on HUEVC angiogenesis in vitro was detected by cell scratch test and in vitro angiogenesis technique. It was found that o-phenanthrene Up linoselenediazole inhibited the migration of HUEVC cells in a dose-dependent manner. It was further found that with the increase of the concentration of o-phenanthrene Up linoselenediazole, at 4-6 渭 M, the vasculoid structure became shorter and incomplete than that of the blank control group, and the tubular results disintegrated under the action of 8 渭 M SePhen. Cell apoptosis increased. 2 mice models of low selenium, suitable selenium and high selenium were established under strict selenium nutrition control. (BMDM), from bone marrow macrophages with different selenium status were isolated and cultured with 80160320ng/mL selenium concentration respectively. It was found that high selenium nutrition level promoted the production of Ros in activated macrophages, and that BMDM5h was treated with low density lipoprotein (LDL) (LDL). CD36, the main receptor of lipid endocytosis, was down-regulated by RT-PCR with the increase of selenium nutrition level. It was found that the level of CD36mRNA was up-regulated to some extent with the increase of selenium nutrition level, but there was no statistical significance. In addition, no significant effect of selenium on lipid uptake of PPAR 纬 in macrophages was found in either protein level or mRNA level. In conclusion, o-phenanthroline Up linselenediazole inhibited cell migration and angiogenesis at high concentration of 8 渭 M. Sodium selenite at high concentration (320ng/mL) may regulate the degradation of CD36 by redox equilibrium and decrease the endocytosis lipid of macrophages, which has a certain significance for the prevention and treatment of atherosclerosis.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R151
【参考文献】
相关期刊论文 前6条
1 阎玉芹,李健群;硒缺乏与甲状腺激素代谢[J];中国地方病防治杂志;1992年03期
2 钟国赣,李云义,孙晓霞,江岩,陈羽,王小明;缺硒对离体大鼠工作心脏心肌收缩性能的影响[J];中国地方病学杂志;1991年04期
3 姜昕,曾和松,郭毅,周志斌,唐冰杉,李富康;The expression of matrix metalloproteinases-9, transforming growth factor-β1 and transforming growth factor-β receptorⅠ in human atherosclerotic plaque and their relationship with plaque stability[J];Chinese Medical Journal;2004年12期
4 夏敏;白书阁;白大芳;;硒对老龄雄性大鼠机体谷胱甘肽过氧化物酶、过氧化脂质及其比值的影响[J];老年学杂志;1991年01期
5 黄峙,向军俭,郑文杰,郭宝江;钝顶螺旋藻富集转化硒及硒在藻体中的分布[J];植物生理学通讯;2001年01期
6 丁健;抗肿瘤药物的研究新进展[J];中国新药杂志;2000年03期
，本文编号：2234103
本文链接：https://www.wllwen.com/yixuelunwen/yufangyixuelunwen/2234103.html
