酚试剂分光光度法测定水中微量戊二醛

发布时间：2018-09-10 06:24

【摘要】：[目的]建立一种快速有效地检测水中微量戊二醛的酚试剂分光光度法。[方法]采用戊二醛与酚试剂(3-甲基-苯并噻唑腙,MBTH)反应生成嗪,嗪在酸性溶液中能够被高铁离子氧化生成蓝绿色化合物,利用产生的颜色与戊二醛含量成正比的原理,采用酚试剂分光光度法对水中微量戊二醛含量进行测定,分析入射波长、酚试剂浓度、显色剂酸溶解条件、显色温度和显色时间等因素对实验结果的影响并确定最佳条件。[结果]确定了604 nm处为最佳吸收波长;MBTH和硫酸高铁铵的最佳用量分别为200 mg/L和1 800 mg/L;在30℃下放置15 min后MBTH与戊二醛能够充分反应生成嗪;其次研究了显色剂酸溶解条件,其最佳盐酸溶解用量为0.2 mol/L;最后研究得出最佳显色温度和显色时间为40℃下静置40 min。在最佳测试条件下,吸光度与戊二醛浓度在0.005~1.00 mg/L范围内呈现良好线性关系,检测限为0.005 mg/L,相对标准偏差为0.34%,平均加标回收率为98.13%。[结论]该方法检测限低、灵敏度高、线性范围宽,能够快速有效地检测水中微量戊二醛。
[Abstract]:[objective] to establish a rapid and effective spectrophotometric method for the determination of trace glutaraldehyde in water. [methods] the reaction of glutaraldehyde with phenol reagent (3-methyl-benzothiazolyl Hydrazone MBTH) was used to produce pyrazine, which could be oxidized by iron ion in acid solution to form blue-green compound. The color produced was proportional to the content of glutaraldehyde. The content of glutaraldehyde in water was determined by phenol reagent spectrophotometry. The influence of incident wavelength, concentration of phenol reagent, acid dissolution condition of chromogenic agent, color reaction temperature and color reaction time on the experimental results were analyzed and the optimum conditions were determined. [results] the optimum absorption wavelength at 604 nm was determined to be 200 mg/L and 1 800 mg/L;, respectively. MBTH and glutaraldehyde could fully react with glutaraldehyde at 30 鈩，

本文编号：2233650