WSC-DTPA纳米粒辐射防护作用的初步研究

发布时间：2018-09-12 09:08

【摘要】：为研究WSC-DTPA(水溶性低分子量壳聚糖WSC,二乙烯三胺五乙酸DTPA)纳米粒的辐射防护作用,采用N-乙酰化反应和离子凝胶法制备不同游离氨基含量的WSC-DTPA纳米粒;MTT法检测其对6 Gy60Coγ射线照射后48 h BRL细胞存活率的影响;活细胞工作站观察BRL细胞摄取FITC-WSC-DTPA纳米荧光探针的情况。结果表明:成功合成了游离氨基含量分别为92.7%、74.3%、1.59%的WSC-DTPA聚合物;WSC、WSC纳米粒以及WSC-DTPA纳米粒(氨基含量为92.7%,浓度在6.25μg/mL以上),随着药物浓度的增加,BRL细胞存活率均显著高于单纯照射组,差别有统计学意义(p0.05),而游离氨基含量为1.59%的WSC-DTPA纳米粒无辐射保护作用;活细胞工作站检验结果显示2 h内WSC纳米粒、WSC-DTPA纳米粒能够进入BRL细胞,而非纳米化的WSC-DTPA聚合物无法进入细胞。
[Abstract]:In order to study the radiation protection of WSC-DTPA (water-soluble low molecular weight chitosan WSC, diethylenetriamine pentaacetic acid DTPA) nanoparticles, WSC-DTPA nanoparticles with different free amino groups were prepared by N-acetylation reaction and ionic gel method. The effects of WSC-DTPA nanoparticles on the survival rate of BRL cells 48 h after irradiation with 6 Gy60Co 纬 -rays were measured. Live cell workstation was used to observe the uptake of FITC-WSC-DTPA nanofluorescent probe by BRL cells. The results showed that the WSC-DTPA polymer WSC nanoparticles and WSC-DTPA nanoparticles with a free amino group of 92.74.3and 1.59% were successfully synthesized (the amino content was 92.7and the concentration was above 6.25 渭 g/mL). With the increase of the drug concentration, the survival rate of WSC-DTPA cells was significantly higher than that of the irradiation group. The difference was statistically significant (p0.05), but the free amino content of 1.59% WSC-DTPA nanoparticles had no radiation protection. The results of living cell workstation test showed that WSC nanoparticles WSC-DTPA nanoparticles could enter BRL cells within 2 hours. The non-nanocrystalline WSC-DTPA polymer does not enter the cell.
【作者单位】：苏州大学医学部放射医学与防护学院江苏省放射医学与防护重点实验室;苏州大学医学部实验中心;
【基金】：江苏高校省级重点实验室开放研究课题(KJS0829) 江苏省优势学科资助
【分类号】：R142

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