四种饮用水对小鼠肝肾功能及脏器锌铁含量的影响

发布时间：2018-10-08 09:36

【摘要】：目的探讨4种饮用水对小鼠肝肾功能及各脏器锌铁含量的影响。方法分别测定纯水、自来水、矿泉水和过滤水中锌、铁浓度。将80只清洁级ICR小鼠随机分为4组,每组20只,雌雄各半,分别用纯水(对照组)、自来水、矿泉水和过滤水喂养,90 d后眼球采血,测定小鼠血清谷丙转氨酶(ALT)、总蛋白(TP)、尿素氮(BUN)、尿酸(UA)及肌酐(Cr)含量,随后立即处死小鼠,取肝脏、肾脏、心脏、胰腺及脑测定锌、铁含量,并制作肝肾组织石蜡切片观察其形态学变化。结果过滤水组小鼠血清Cr含量[(36.00±8.83)μmol/L]明显低于纯水组、自来水组及矿泉水组[(44.83±12.64)、(44.56±10.52)、(43.79±10.53)μmol/L],差异有统计学意义(P0.05);纯水组小鼠肝脏、肾脏锌含量[(33.17±6.18)、(16.69±8.12)μg/g]明显低于过滤水组[(45.31±7.32)、(22.61±6.01)μg/g],差异有统计学意义(P0.05);小鼠血清Cr与肾脏锌含量呈显著负相关关系(r=-0.300,P0.05);不同组小鼠血清ALT、TP、BUN、UA及各脏器内铁含量差异均无统计学意义,同时肝肾组织结构未见异常。结论长期饮用过滤水有利于肾小球滤过屏障功能的发挥,其可能与肾锌水平提高有关。
[Abstract]:Objective to study the effects of four kinds of drinking water on liver and kidney function and zinc and iron content in various organs of mice. Methods the concentrations of zinc and iron in pure water, tap water, mineral water and filtered water were determined respectively. 80 ICR mice of clean grade were randomly divided into 4 groups, 20 rats in each group, half male and half female. Blood was collected from eyeball after feeding with pure water (control group), tap water, mineral water and filtered water for 90 days. Serum glutamate-alanine aminotransferase (ALT),) total protein (TP), urea nitrogen (TP), urea-nitrogen (BUN), uric acid (UA) and creatinine (Cr) were measured. The mice were killed immediately. The contents of zinc and iron in liver, kidney, heart, pancreas and brain were measured. Paraffin sections of liver and kidney were made to observe the morphological changes. Results the content of serum Cr in the filtered water group [(36.00 卤8.83) 渭 mol/L] was significantly lower than that in the pure water group and the tap water group and mineral water group [(44.83 卤12.64), (44.56 卤10.52), (43.79 卤10.53) 渭 mol/L] (P0.05). The content of zinc in kidney [(33.17 卤6.18), (16.69 卤8.12) 渭 g / g] was significantly lower than that in filtered water group [(45.31 卤7.32), (卤22.61 卤6.01) 渭 g / g], the difference was significant (P0.05), and there was a significant negative correlation between serum Cr and renal zinc content (r-0.300P0.05). At the same time, there was no abnormal structure of liver and kidney. Conclusion drinking filtered water for a long time is beneficial to play the function of glomerular filtration barrier, which may be related to the increase of zinc level in kidney.
【作者单位】：福建医科大学卫生检验与检疫学系;福建医科大学预防医学实验中心;
【基金】：福建省科技计划重大项目(No.2012Y4004)
【分类号】：R123.5

【参考文献】