氯仿和邻苯二甲酸二辛酯联合染毒对小鼠肝脏酶的影响

发布时间：2018-10-08 09:14

【摘要】：目的观察氯仿(chloroform,CF)和邻苯二甲酸二辛酯(dioctylphthalate,DOP)联合染毒对小鼠肝脏酶的影响。方法选取40只成年ICR小鼠,随机分成8组,即雌性对照组(玉米油)、雌性DOP单独染毒组(1/10 LD_(50),540 mg/kg)、雌性CF单独染毒组(1/10 LD_(50),112 mg/kg)、雌性DOP(1/10 LD_(50),540 mg/kg)+CF(1/10 LD_(50),112 mg/kg)联合染毒组,雄性也做同样分组及处理,每组5只。采用灌胃方式连续染毒15 d,每天1次。测定肝脏组织中乳酸脱氢酶(LDH)、ATP酶活力。结果与对照组比较,DOP组、CF组和联合染毒组小鼠肝脏器系数、LDH活力均升高,Na~+-K~+-ATP酶、Ca~(2+)-Mg~(2+)-ATP酶活力均降低,差异有统计学意义(P0.05)。雄性联合染毒组肝脏器系数均高于DOP单独染毒组、CF单独染毒组,差异有统计学意义(P0.05);雌性联合染毒组与DOP单独染毒组的肝脏器系数无明显差异(P0.05),高于CF单独染毒组,差异有统计学意义(P0.05)。与DOP单独染毒组比较,联合染毒组雌性小鼠的LDH、Na~+-K~+-ATP酶活力均无明显差异(P0.05),Ca~(2+)-Mg~(2+)-ATP酶活力升高,差异有统计学意义(P0.05);与CF单独染毒组比较,联合染毒组雌性小鼠LDH活力升高,Ca~(2+)-Mg~(2+)-ATP酶活力下降,差异均有统计学意义(P0.05),Na~+-K~+-ATP酶活力无明显差异(P0.05)。与DOP单独染毒组比较,联合染毒组雄性小鼠的Ca~(2+)-Mg~(2+)-ATP、Na~+-K~+-ATP酶活力均无明显差异(P0.05),LDH酶活力升高,差异有统计学意义(P0.05);与CF单独染毒组比较,联合染毒组雄性小鼠的LDH活力升高,Na~+-K~+-ATP酶、Ca~(2+)-Mg~(2+)-ATP酶活力下降,差异均有统计学意义(P0.05)。雄性联合染毒对肝脏器系数的影响尚未表现出联合作用(P0.05),其他各联合组均存在联合作用(P0.05),表现为拮抗作用。结论DOP和CF联合染毒可能对小鼠肝脏系统的无氧糖酵解和能量代谢产生抑制作用。
[Abstract]:Objective to observe the effect of chloroform (chloroform,CF) combined with dioctyl phthalate (dioctylphthalate,DOP) on liver enzymes in mice. Methods Forty adult ICR mice were randomly divided into 8 groups: female control group (corn oil), female DOP alone (1 / 10 LD_ (50) 540 mg/kg), female CF alone (1 / 10 LD_ (50) 1 / 112 mg/kg) and female DOP (1 / 10 LD_ (50) 540mg/kg) CF (1 / 10 LD_ (50) 112mg/kg). The males were also divided into groups and treated with 5 rats in each group. The rats were administrated by gavage for 15 days, once a day. The activity of lactate dehydrogenase (LDH) ATPase in liver tissue was measured. Results compared with the control group, the liver organ coefficient and LDH activity in the DOP group and the combined exposure group were increased, and the activities of Na ~ (2) -mg ~ (2) -ATPase decreased significantly (P0.05). The liver organ coefficient of male combined exposure group was higher than that of DOP alone group (P0.05), and the liver organ coefficient of female combined exposure group and DOP alone group had no significant difference (P0.05), which was higher than that of CF alone group. The difference was statistically significant (P0.05). There was no significant difference in the activity of LDH,Na~ -K ~ -ATPase in female mice exposed to DOP alone (P0.05), the activity of Ca2 -Mg2 -ATPase increased significantly (P0.05), and the activity of LDH,Na~ -K ~ -ATPase was significantly higher than that of CF alone (P0.05), and compared with that of CF alone, the activity of LDH,Na~ -K ~ (2) -ATPase in the combined group was significantly higher than that in the control group (P0.05). The LDH activity of female mice in combined exposure group increased and the activity of Ca ~ (2) -mg ~ (2) -ATPase decreased, there was no significant difference (P0.05) in Na ~ -K ~ -ATPase activity (P0.05). There was no significant difference in Ca~ (2) -mg ~ (2) -ATPase activity of Na- K ~ -ATPase between the combined exposure group and the DOP alone group (P0.05), the difference was statistically significant (P0.05), and compared with the CF alone group, there was no significant difference in Ca~ _ (2) -mg ~ (2) -ATPase activity between the two groups. The LDH activity of the combined exposure group increased with the decrease of Ca ~ (2) -mg ~ (2) -ATPase activity of Na ~ -K ~ -ATPase (P0.05). The effect of male combined exposure on liver organ coefficient has not yet shown a combined effect (P0.05), other combined groups have combined effects (P0.05), showing antagonism. Conclusion combined exposure of DOP and CF may inhibit anaerobic glycolysis and energy metabolism in mouse liver system.
【作者单位】：齐齐哈尔医学院齐齐哈尔医学科学院;大庆市人民医院;吉林大学公共卫生学院;齐齐哈尔技师学院;
【基金】：齐齐哈尔医学院院内科研基金(QY2016Q-07)
【分类号】：R114

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