魔芋低聚糖对三硝基苯磺酸致小鼠溃疡性结肠炎的保护作用

发布时间：2018-10-09 07:52

【摘要】：目的:初步探讨魔芋低聚糖(KOGM)对三硝基苯磺酸(TNBS)诱导的小鼠溃疡性结肠炎的保护作用。方法:将昆明小鼠随机分为空白组、模型组、KOGM低剂量组和高剂量组。用100mg/kg bw TNBS和50%乙醇混合溶液造模。自造模24h后每天给小鼠灌胃KOGM直至处死。灌胃体积为10m L/kg bw。结果:各剂量组每日平均摄食量无显著性差异,但与模型组比较,低、高剂量组食物利用率和体重增重均显著升高;与模型组比较,低剂量组和高剂量组小鼠的结肠溃疡面积及溃疡率均明显降低(p0.01);结肠组织病理切片结果表明,KOGM高剂量组小鼠受损结肠恢复较好甚至接近正常状态。结论:KOGM对小鼠溃疡性结肠炎有一定的保护作用,其作用机理需进一步研究。
[Abstract]:Aim: to investigate the protective effect of konjac oligosaccharide (KOGM) on mice ulcerative colitis induced by trinitrobenzene sulfonic acid (TNBS). Methods: Kunming mice were randomly divided into blank group, model group, low dose group and high dose group. The model was made with 100mg/kg bw TNBS and 50% ethanol mixed solution. After 24 hours, mice were given KOGM daily until they were killed. The volume of gastric perfusion is 10 m L/kg bw.. Results: there was no significant difference in the average daily intake of each dose group, but compared with the model group, the food utilization rate and weight gain in the low and high dose groups were significantly increased, and compared with the model group, the food utilization rate and body weight gain of the model group were significantly higher than that of the model group. The colonic ulcer area and ulceration rate of mice in low dose group and high dose group were significantly decreased (p0.01), and the results of colonic histopathology showed that the damaged colon recovered well or close to the normal state in the high dose group (P < 0.05). Conclusion: Kogm has a protective effect on ulcerative colitis in mice, and its mechanism needs further study.
【作者单位】：北京联合大学应用文理学院生物活性物质与功能食品北京市重点实验室;
【基金】：“十二五”国家科技支撑计划课题资助(2012BAD33B06) 北京联合大学“启明星”大学生科技创新项目(市级)资助
【分类号】：R151;TS201.4

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