骨髓间充质干细胞移植对矽肺大鼠肺纤维化的作用
发布时间：2018-10-20 08:43
【摘要】：目的 探讨骨髓间充质干细胞(BMSCs)移植对矽肺大鼠肺组织纤维化的影响。方法 将42只SD雌性大鼠分为A、B、C、D组。A、B和C组采用暴露式气管内一次性缓慢注入二氧化硅(SiO2)混悬液建立矽肺大鼠模型,D组则注入生理盐水。B、C组分别于造模后6h或第42天经尾静脉注射BMSCs。第14天,A、B和D组分别处死大鼠各6只,第56天各组分别处死6只。HE染色观察肺组织形态学变化,ELISA检测肺组织羟脯氨酸(Hyp)水平。结果 A组在造模后第14天,肺组织病理改变以急性炎症反应为主,第56天以纤维化为主;B、C组病理改变均较同时期A组轻;D组未见明显异常。肺组织中Hyp水平,A、B和C组均较D组明显升高(P=0.000);B、C组与A组比较,在BMSCs治疗后第14天均明显减低(P=0.000、0.035),B组在治疗后第56天仍明显减低(P=0.016)。结论 不论在以肺泡炎为主还是在以纤维化为主的病理阶段予BMSCs移植,均可以延缓矽肺大鼠肺纤维化的病理进程。
[Abstract]:Objective to investigate the effect of bone marrow mesenchymal stem cell (BMSCs) transplantation on pulmonary fibrosis in silicosis rats. Methods Forty-two female SD rats were divided into two groups: group A (B) and group C (group C) were injected with exposed intratracheal silica (SiO2) suspension, and group D (group D) were injected with normal saline, respectively, 6 hours or the first time after the establishment of the model. 42 days BMSCs. via caudal vein On the 14th day, 6 rats were killed in group A B and 6 rats in group D, 6 rats in each group were killed on the 56th day. Morphologic changes of lung tissue were observed by HE staining and hydroxyproline (Hyp) level of lung tissue was detected by ELISA. Results the pathological changes of lung tissue in group A were mainly acute inflammatory reaction and fibrosis on the 14th day after modeling, the pathological changes in group B C were lighter than those in group A at the same time, and no obvious abnormality was found in group D. The level of Hyp in lung tissue in group A B and C was significantly higher than that in group D (P0. 000), and that in group C was significantly lower than that in group A on the 14th day after treatment (P < 0. 0000. 035), B) (P0. 016). Conclusion BMSCs transplantation can delay the pathological process of pulmonary fibrosis in silicosis rats.
【作者单位】： 广东省深圳市职业病防治院职业病科;
【分类号】：R322.3
[Abstract]:Objective to investigate the effect of bone marrow mesenchymal stem cell (BMSCs) transplantation on pulmonary fibrosis in silicosis rats. Methods Forty-two female SD rats were divided into two groups: group A (B) and group C (group C) were injected with exposed intratracheal silica (SiO2) suspension, and group D (group D) were injected with normal saline, respectively, 6 hours or the first time after the establishment of the model. 42 days BMSCs. via caudal vein On the 14th day, 6 rats were killed in group A B and 6 rats in group D, 6 rats in each group were killed on the 56th day. Morphologic changes of lung tissue were observed by HE staining and hydroxyproline (Hyp) level of lung tissue was detected by ELISA. Results the pathological changes of lung tissue in group A were mainly acute inflammatory reaction and fibrosis on the 14th day after modeling, the pathological changes in group B C were lighter than those in group A at the same time, and no obvious abnormality was found in group D. The level of Hyp in lung tissue in group A B and C was significantly higher than that in group D (P0. 000), and that in group C was significantly lower than that in group A on the 14th day after treatment (P < 0. 0000. 035), B) (P0. 016). Conclusion BMSCs transplantation can delay the pathological process of pulmonary fibrosis in silicosis rats.
【作者单位】： 广东省深圳市职业病防治院职业病科;
【分类号】：R322.3
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