不同养殖场家蝇成虫体表携带细菌的分离与鉴定

发布时间：2018-10-23 07:30

【摘要】：目的研究不同养殖场内家蝇成虫体表携带细菌数量和类别。方法采用网捕法对不同养殖场的家蝇进行采样,利用振荡洗脱法分离其体表细菌,对获得细菌的16SrDNA基因扩增后进行分离分子鉴定。结果不同养殖场内家蝇密度、体表携带细菌数量和种类有统计学差异,且家蝇密度与体表携带细菌数量和携带的细菌属数呈线性相关关系。在养殖场内分离到的家蝇体表携带细菌中,葡萄球菌属、克雷伯氏菌属、埃希菌属、沙雷氏菌属分布较广,同时分离的细菌中多为条件致病菌,存在疾病传播风险。结论养殖场家蝇密度反映出养殖场的整体管理水平;养殖场孳生的家蝇可机械性携带条件致病菌,存在将细菌传播给人类的风险,需要进一步加强对养殖场蝇类防控及携带细菌的监控。
[Abstract]:Objective to study the number and types of bacteria carried by housefly adults in different farms. Methods Musca domestica in different farms were sampled by net trapping method. The bacteria on the body surface were isolated by oscillatory elution method. The 16SrDNA gene of the bacteria was amplified and identified by molecular isolation. Results there were significant differences in the density of Musca domestica and the number and species of bacteria carried on the body surface in different farms, and there was a linear correlation between the density of Musca domestica and the number of bacteria carried on the surface and the number of bacteria belonging to the body surface. Staphylococcus, Klebsiella, Escherichia coli and Shareh are the most common bacteria isolated from housefly. Conclusion the density of housefly in farm reflects the whole management level of farm, the housefly breeding in farm can carry conditional pathogenic bacteria mechanically, and there is the risk of transmitting bacteria to human beings. It is necessary to further strengthen the control of flies and the control of bacteria in farms.
【作者单位】：泰山医学院新发传染病溯源及防控协同创新中心;泰山医学院基础医学院;
【基金】：国家自然科学基金(Nos.81401693,81572028,81271874) 山东省科技发展计划项目(No.2014GSF121007)联合资助~~
【分类号】：R184.3

【相似文献】