含重组人乳铁蛋白乳粉蛋白质营养学评价

发布时间：2018-10-30 15:29

【摘要】：目的评价牛乳腺生物反应器表达的重组人乳蛋白乳粉蛋白质的营养价值。方法选用100只清洁级刚断乳(出生后20~23 d)SD大鼠(雌、雄各半),按性别、体质量随机分为5组:重组人乳蛋白乳粉组、普通乳粉组、酪蛋白乳粉组、牛乳铁蛋白乳粉组、重组人乳铁蛋白乳粉组,分别喂饲相应饲料28 d,观察大鼠的进食量、体质量,测定血常规、血生化情况,试验末期处死动物,称量脏器重量并对脏器进行病理学检查,计算蛋白功效比和校正蛋白功效比,对氨基酸评分。结果重组人乳铁蛋白乳粉雄性动物组总体蛋白功效比值差异有统计学意义(P0.05),但重组人乳铁蛋白乳粉组雄性和其他对照组差异无统计学意义(P0.05)。雌性动物重组人乳铁蛋白乳粉组和其他对照组蛋白功效比值差异无统计学意义(P0.05)。结论重组人乳铁蛋白乳粉的蛋白功效和其他组相比并无较大区别,氨基酸评分结果显示苯丙氨酸和酪氨酸为第一限制氨基酸,与普通乳粉一样。
[Abstract]:Objective to evaluate the nutritional value of recombinant human milk protein expressed in bovine mammary gland bioreactor. Methods 100 freshly weaned rats (20: 23 d) SD postnatal) were randomly divided into 5 groups according to their sex and body weight: recombinant human milk powder group, ordinary milk powder group, casein milk powder group, bovine lactoferrin milk powder group. The rats in the recombinant human lactoferrin milk powder group were fed with the corresponding feed for 28 days. The rats were observed for food intake, body weight, blood routine, blood biochemistry, the animals were killed at the end of the experiment, the weight of organs was weighed and the viscera were examined by pathology. Calculate protein efficacy ratio and corrected protein efficacy ratio, and score amino acids. Results there was significant difference in the ratio of total protein efficacy between the male group and the control group (P0.05), but there was no significant difference between the male group and the other control groups (P0.05). There was no significant difference in the ratio of protein efficacy between female animal recombinant human lactoferrin milk powder group and other control group (P0.05). Conclusion the effect of recombinant human lactoferrin milk powder is not different from that of other groups. The results of amino acid score show that phenylalanine and tyrosine are the first limiting amino acids, which is the same as ordinary milk powder.
【作者单位】：中国疾病预防控制中心营养与健康所卫生计生委微量元素营养重点实验室;
【基金】：转基因生物的食用和饲用安全评价技术(2014ZX08001-005)
【分类号】：R151

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