不同剂量紫苏油对大鼠血脂水平影响

发布时间：2018-10-31 13:08

【摘要】：目的探讨不同剂量紫苏油对大鼠血脂水平和内脏脂肪含量影响。方法 40只Wistar雄性大鼠随机分为5组:对照组、高脂组及紫苏油低、中、高剂量组(6%、10%、15%),饲以相应的配方饲料8周;以酶比色法测定大鼠血清总胆固醇(TC)和甘油三酯(TG)水平,聚乙烯硫酸沉淀法测定低密度脂蛋白胆固醇(LDL-C)水平,磷钨酸-镁沉淀法测定高密度脂蛋白胆固醇(HDL-C)水平;取肝脏,分离双侧肾脏周围、附睾周围脂肪垫,分别称重。结果与对照组比较,高脂组大鼠血清TC水平[(1.90±0.37)mmol/L]升高(P0.05);与高脂组比较,紫苏油低、中、高剂量组大鼠血清TC水平[分别为(1.54±0.36)、(1.22±0.14)、(1.34±0.22)mmol/L]均降低(P0.05);与高脂组大鼠血清TG和HDL-C水平[分别为(0.65±0·11)、(0.77±0.12)mmol/L]比较,高剂量紫苏油组大鼠TG和HDL-C水平[分别为(0.44±0.11)、(0.56±0.14)mmol/L]均降低(P0.05);与对照组比较,高脂组及紫苏油低、中、高剂量组大鼠肝系数均升高(P0.05)。结论紫苏油可降低高脂大鼠血清TC水平,但当饲料中紫苏油含量过高时(15%)也会降低大鼠血清HDL-C水平。
[Abstract]:Objective to investigate the effects of different doses of perilla oil on blood lipid level and visceral fat content in rats. Methods Forty male Wistar rats were randomly divided into 5 groups: control group, high fat group, low perilla oil, middle and high dose group (61010%), fed with the corresponding formula for8 weeks. Serum total cholesterol (TC) and triglyceride (TG) levels were measured by enzyme colorimetry, and low density lipoprotein cholesterol (LDL-C) levels were measured by polyethylene sulphuric acid precipitation method. High density lipoprotein cholesterol (HDL-C) was determined by phosphotungstic acid-magnesium precipitation method. The liver was taken and the fat pads around the kidney and epididymis were separated and weighed respectively. Results compared with the control group, the serum TC level in the hyperlipidemia group [(1.90 卤0.37) mmol/L] was increased (P0.05). Compared with the high-fat group, the serum TC level in the perilla oil group was lower than that in the high-fat group [(1.54 卤0.36), () 1.22 卤0.14), (1.34 卤0.22 mmol/L] (P0.05). Compared with the serum TG and HDL-C levels of the hyperlipidemic rats [(0.65 卤0.11), (卤0.77 卤0.12) mmol/L, respectively], the levels of TG and HDL-C in the high-dose perilla oil group were (0.44 卤0.11), and those in the high-dose perilla oil group were (0.44 卤0.11), respectively. (0. 56 卤0. 14) mmol/L] decreased (P0.05); Compared with the control group, the liver coefficients of the rats in the high fat group and perilla oil group were higher than those in the control group (P0.05). Conclusion Perilla oil can decrease the level of serum TC in hyperlipidemic rats, but when the content of perilla oil in feed is too high (15%), it can also decrease the level of serum HDL-C in rats.
【作者单位】：中国医科大学公共卫生学院营养与食品卫生教研室;中国医科大学附属第一医院临床营养科;
【分类号】：R151

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