铅调控组蛋白乙酰化对大鼠心血管细胞增殖及凋亡影响

发布时间：2018-11-01 11:12

【摘要】：目的：观察铅暴露对大鼠心血管细胞的增殖、凋亡的影响，探讨铅对心血管细胞增殖和凋亡影响的分子机制。方法：选取健康，3-4周龄的SD大鼠52只，体重在99-114g。使用随机数字法将SD大鼠分为正常对照组和实验组，实验组饮用1％的乙酸铅水，正常对照组只饮用自来水，12天后处死正常对照组和实验组各13只，取心脏和血管组织备用。40天后处死正常对照组和实验组各13只，取心脏和血管组织备用。提取组织细胞总蛋白和核蛋白，使用Westernblot法检测铅暴露对心血管细胞增殖、凋亡和组蛋白乙酰化的变化情况。使用用凝胶图象分析系统进行X光片扫描，法国Vilber Lourmat公司的Bio ID数码成像分析软件对Western印迹结果进行定量分析。数据使用SPSS13.0统计软件进行数据分析，应用t检验分析。结果： 1铅暴露不同时间大鼠心脏组织细胞增殖核抗原PCNA、凋亡相关基因Bcl-2和Bax以及组蛋白H3乙酰化的表达变化 Western blot和计算机western blot图像半定量分析结果显示，与正常对照组比较，铅暴露12天和40天的大鼠心脏组织细胞中增殖蛋白PCNA的表达明显增加，差异具有统计学意义。铅暴露12天后SD大鼠心脏组织细胞中的Bcl-2蛋白表达量明显增加，Bax蛋白表达增加；铅暴露喂养40天后的SD大鼠心脏组织细胞中Bcl-2蛋白表达量明显减少，Bax蛋白表达增加，差异均具有统计学意义。铅暴露12天和40天的大鼠心脏组织细胞中组蛋白H3乙酰化的表达明显增加，具有统计学差异。 2铅暴露不同时间的大鼠主动脉血管细胞中增殖核抗原PCNA、凋亡相关基因Bcl-2和Bax以及组蛋白H3乙酰化的表达变化 Western blot和计算机western blot图像半定量分析结果显示，与正常对照组比较，铅暴露12天和40天的后大鼠主动脉血管细胞中的PCNA蛋白表达量明显减少，差异具有明显的统计学意义。铅暴露12天的SD大鼠主动脉血管细胞中Bcl-2蛋白表达量明显减少，Bax蛋白表达量减少；铅暴露40天的SD大鼠主动脉血管细胞中Bcl-2蛋白表达量减少，Bax蛋白的表达量增加，差异均具有统计学意义。铅暴露12天和40天后的大鼠主动脉血管细胞中乙酰化的组蛋白H3表达量明显增加，具有统计学差异。结论： 1铅暴露使心脏组织细胞出现增殖、凋亡和组蛋白H3乙酰化水平的升高，，提示铅暴露过程中组蛋白H3乙酰化在心脏组织细胞的增殖和凋亡中起着重要作用。 2铅暴露抑制了血管组织细胞的增殖，促进了血管组织细胞的凋亡和组蛋白H3的乙酰化，说明铅暴露的过程中组蛋白H3乙酰化在血管组织细胞的增殖和凋亡过程中起着重要作用。
[Abstract]:Aim: to observe the effects of lead exposure on the proliferation and apoptosis of cardiovascular cells in rats and to explore the molecular mechanism of the effects of lead on the proliferation and apoptosis of cardiovascular cells. Methods: 52 healthy 3-4-week old SD rats with body weight of 99-114 g were selected. SD rats were randomly divided into control group and experimental group. The experimental group drank 1% lead acetate water, and the normal control group only drank tap water. After 12 days, 13 normal control group and 13 experimental group were killed. After 40 days, the normal control group and the experimental group were killed. Total protein and nuclear protein were extracted and the changes of proliferation apoptosis and histone acetylation of cardiovascular cells exposed to lead were detected by Westernblot method. The gel image analysis system was used to scan X-ray film and the Bio ID digital imaging analysis software of Vilber Lourmat Company of France was used to quantitatively analyze the results of Western blotting. The data are analyzed by SPSS13.0 statistical software and t-test. Results: 1 the changes of expression of apoptosis-related genes Bcl-2 and Bax and histone H3 acetylation of proliferating nuclear antigen (PCNA,) in rat heart tissue at different time after exposure to lead were detected by semi-quantitative analysis of Western blot and western blot images. Compared with the control group, the expression of proliferating protein PCNA in cardiac tissue cells of rats exposed to lead for 12 days and 40 days was significantly increased, and the difference was statistically significant. After 12 days of lead exposure, the expression of Bcl-2 protein and Bax protein in cardiac tissue cells of SD rats were significantly increased. After 40 days of lead exposure, the expression of Bcl-2 protein decreased significantly and the expression of Bax protein increased in the heart of SD rats. The difference was statistically significant. The expression of histone H 3 acetylated in cardiac tissue cells of rats exposed to lead for 12 days and 40 days was significantly increased with statistical difference. 2 the changes of expression of proliferating nuclear antigen PCNA, apoptosis-related genes Bcl-2 and Bax and histone H3 acetylation in rat aortic vascular cells exposed to lead at different time. The results of semi-quantitative analysis of Western blot and computer western blot images showed that: 1. Compared with the control group, the expression of PCNA protein in aortic vascular cells of rats exposed to lead for 12 days and 40 days was significantly decreased, and the difference was statistically significant. The expression of Bcl-2 protein and Bax protein in aortic vascular cells of SD rats exposed to lead for 12 days decreased significantly. After 40 days of lead exposure, the expression of Bcl-2 protein decreased and the expression of Bax protein increased in aortic vascular cells of SD rats. The expression of acetylated histone H3 in aortic vascular cells of rats exposed to lead for 12 days and 40 days was significantly increased with statistical difference. Conclusion: 1 lead exposure induced proliferation, apoptosis and histone H _ 3 acetylation, suggesting that histone H _ 3 acetylation plays an important role in the proliferation and apoptosis of cardiac tissue cells during lead exposure. 2 lead exposure inhibited the proliferation of vascular tissue cells, promoted the apoptosis of vascular tissue cells and the acetylation of histone H3. These results suggest that histone H 3 acetylation plays an important role in the proliferation and apoptosis of vascular tissue cells during lead exposure.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114

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