利用糖芯片技术快速检测沙门氏菌

发布时间：2018-11-07 08:09

【摘要】：为了探讨糖芯片在沙门氏菌检测上的应用,以沙门氏菌ATCC31685为主要研究对象,制作不同类型的糖芯片用于优化糖芯片检测条件;在优化条件的基础上,降低糖浓度或细菌浓度用于确定糖芯片检测限值;继而系统地研究了糖芯片在沙门氏菌和凝集素FimH拮抗剂筛选上的应用,并探究糖芯片对菌株的破坏性。由实验现象可知,沙门氏菌可与甘露糖化合物修饰的糖芯片结合,其中,以Man-1与NHS玻片所构建的糖芯片效果最优,并且两种不同的沙门氏菌(ATCC31685和ATCC9184)与糖芯片的结合能力不同,因此糖芯片可作为沙门氏菌的一种快速检测手段;在糖浓度为313 mmol/L或细菌浓度106个/mL时荧光信号接近背景值,并且糖芯片检测后对菌株无破坏性,可继续其他检测项目;此外,实验结果表明糖芯片可作为FimH蛋白拮抗剂的筛选工具。
[Abstract]:In order to explore the application of sugar chip in the detection of salmonella, different types of sugar chips were made to optimize the detection conditions of the sugar chip, taking Salmonella ATCC31685 as the main research object. On the basis of optimizing conditions, reducing sugar concentration or bacterial concentration was used to determine the detection limit of sugar chip, and then the application of sugar chip in screening salmonella and lectin FimH antagonist was systematically studied, and the destructive effect of sugar chip on the strain was explored. According to the experimental phenomenon, salmonella can bind to sugar chip modified by mannose compound, and the sugar chip made of Man-1 and NHS glass is the best. And two different Salmonella (ATCC31685 and ATCC9184) have different binding ability with sugar chip, so sugar chip can be used as a rapid detection method of Salmonella. When sugar concentration is 313 mmol/L or bacterial concentration is 106 / mL, the fluorescence signal is close to background value, and the detection of sugar chip has no damage to the strain, so it can continue other detection items. In addition, the results showed that the sugar chip could be used as a screening tool for FimH protein antagonists.
【作者单位】：江南大学生物工程学院糖化学与生物技术教育部重点实验室;江南大学无锡医学院;
【基金】：江苏省自然科学基金项目(BK20150140,BK20140154) 江南大学公共卫生中心重点项目(JUPH201502)
【分类号】：R155.5

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