基于近红外免疫层析技术的食源性副溶血弧菌快速检测方法研究

发布时间：2018-11-07 09:14

【摘要】：建立一种应用近红外荧光免疫层析技术快速检测副溶血弧菌的方法。采用近红外荧光标记副溶血弧菌单克隆抗体,将副溶血弧菌多克隆抗体和羊抗鼠IgG多克隆抗体喷涂于硝酸纤维膜上分别作为检测线和质控线,研制检测副溶血弧菌的近红外免疫层析试纸条及配套标准物质。研究表明,所建立的近红外免疫法对检测副溶血弧菌具有良好的特异性和较高的灵敏度,最低检测限为1.2×10~2 CFU/mL,与沙门氏菌、金黄色葡萄球菌、大肠埃希氏菌、单增李斯特菌无交叉反应。通过效果评价试验发现,与传统检测方法相比较,所建立的近红外荧光法检测时间时间最短,检测限与RT-PCR法接近。近红外荧光法在45 min内即可完成检测,可用于食品中副溶血弧菌的高效检测,为食品安全监管提供可靠的技术支持。
[Abstract]:A method for rapid detection of Vibrio parahaemolyticus by near infrared fluorescence immunochromatography was established. The polyclonal antibody of Vibrio parahaemolyticus and the polyclonal antibody of sheep anti-mouse IgG were sprayed on the membrane of nitric acid fiber as the detection line and the quality control line, respectively, using near-infrared fluorescence labeling monoclonal antibody against Vibrio parahaemolyticus. A near-infrared immunochromatographic strip for the detection of Vibrio parahaemolyticus was developed. The results showed that the established near infrared immunoassay had good specificity and high sensitivity for the detection of Vibrio parahaemolyticus. The lowest detection limit was 1.2 脳 10 ~ 2 CFU/mL, with Salmonella, Staphylococcus aureus, Escherichia coli. Listeria monocytogenes had no cross reaction. Compared with the traditional detection method, the established near infrared fluorescence method has the shortest detection time and the detection limit is close to that of RT-PCR method. Near-infrared fluorescence method can be used to detect Vibrio parahaemolyticus in food in 45 min. It can provide reliable technical support for food safety supervision.
【作者单位】：北京出入境检验检疫局检验检疫技术中心;出入境食品安全检测北京市重点实验室;常德出入境检验检疫局;天津出入境检验检疫局工业产品安全技术中心;
【基金】：质检公益性行业科研专项项目(201410049) 国家质检总局科技计划项目(2015IK011)
【分类号】：R155.3

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