纳米二氧化硅对A549细胞的毒性作用
发布时间：2018-11-25 15:46
【摘要】：[目的]研究纳米二氧化硅(nano-SiO_2)对人肺腺癌A549细胞的毒性作用。[方法]将A549细胞随机分成6组,即对照组和实验组(5、10、25、50、100 mg/L nano-SiO_2组)。实验组分别用不同浓度的nano-SiO(210~20 nm粒径)刺激,MTT法检测细胞活性,比色法测定乳酸脱氢酶(LDH)活性,透射电子显微镜观察细胞微观结构,流式细胞仪检测nano-SiO_2对细胞周期以及细胞凋亡的影响。[结果]与对照组相比:随着nano-SiO_2浓度升高,A549细胞存活率呈降低趋势;LDH活性增加(P0.01或P0.05),在浓度为0、5、10、25 mg/L时呈浓度依赖关系,但在nano-SiO_2浓度为50 mg/L时,LDH活力降低,100 mg/L时增高。nano-SiO_2浓度的升高可导致细胞膜表面绒毛凸起减少,细胞器空泡样变增加,次级溶酶体增多;细胞凋亡率增加,坏死率增加;细胞在G0/G1期阻滞,增殖受抑制。[结论]nano-SiO_2作用于A549细胞可被细胞摄取,使细胞器结构功能受损,从而抑制细胞增殖、诱导细胞凋亡,引起细胞周期紊乱。
[Abstract]:[objective] to study the toxic effect of nano silica (nano-SiO_2) on human lung adenocarcinoma cell line A549. [methods] A549 cells were randomly divided into 6 groups, namely control group and experimental group (50 mg/L nano-SiO_2 group). The experimental group was stimulated with different concentrations of nano-SiO (210 ~ 20 nm diameter), the cell activity was detected by MTT method, the activity of lactate dehydrogenase (LDH) was measured by colorimetry, and the microstructure of cells was observed by transmission electron microscope (TEM). The effect of nano-SiO_2 on cell cycle and apoptosis was detected by flow cytometry. [results] compared with the control group, the survival rate of A549 cells decreased with the increase of nano-SiO_2 concentration. The activity of LDH increased (P0.01 or P0.05) in a concentration-dependent manner when the concentration of nano-SiO_2 was 1025 mg/L, but when the concentration of nano-SiO_2 was 50 mg/L, the activity of LDH decreased. At 100 mg/L, the increase of nano-SiO_2 concentration resulted in the decrease of villi bulge, the vacuolation of organelle and the increase of secondary lysosome. Apoptosis rate increased, necrosis rate increased, cell proliferation was inhibited in G0/G1 phase. [conclusion] the effect of nano-SiO_2 on A549 cells can be taken up by the cells and damage the organelle structure and function, thus inhibit cell proliferation, induce apoptosis and cause cell cycle disorder.
【作者单位】： 华北理工大学基础医学院;华北理工大学医学实验研究中心;华北理工大学国家科技部老年医学国际科技合作基地;
【基金】：国家自然科学基金项目(编号:81673119) 华北理工大学大学生创新项目(编号:X2016213) 唐山市老年医学科技创新团队(编号:15130212C)
【分类号】：R114
[Abstract]:[objective] to study the toxic effect of nano silica (nano-SiO_2) on human lung adenocarcinoma cell line A549. [methods] A549 cells were randomly divided into 6 groups, namely control group and experimental group (50 mg/L nano-SiO_2 group). The experimental group was stimulated with different concentrations of nano-SiO (210 ~ 20 nm diameter), the cell activity was detected by MTT method, the activity of lactate dehydrogenase (LDH) was measured by colorimetry, and the microstructure of cells was observed by transmission electron microscope (TEM). The effect of nano-SiO_2 on cell cycle and apoptosis was detected by flow cytometry. [results] compared with the control group, the survival rate of A549 cells decreased with the increase of nano-SiO_2 concentration. The activity of LDH increased (P0.01 or P0.05) in a concentration-dependent manner when the concentration of nano-SiO_2 was 1025 mg/L, but when the concentration of nano-SiO_2 was 50 mg/L, the activity of LDH decreased. At 100 mg/L, the increase of nano-SiO_2 concentration resulted in the decrease of villi bulge, the vacuolation of organelle and the increase of secondary lysosome. Apoptosis rate increased, necrosis rate increased, cell proliferation was inhibited in G0/G1 phase. [conclusion] the effect of nano-SiO_2 on A549 cells can be taken up by the cells and damage the organelle structure and function, thus inhibit cell proliferation, induce apoptosis and cause cell cycle disorder.
【作者单位】： 华北理工大学基础医学院;华北理工大学医学实验研究中心;华北理工大学国家科技部老年医学国际科技合作基地;
【基金】：国家自然科学基金项目(编号:81673119) 华北理工大学大学生创新项目(编号:X2016213) 唐山市老年医学科技创新团队(编号:15130212C)
【分类号】：R114
【参考文献】
相关期刊论文 前4条
1 周子靖;张金波;朱金玲;王淑秋;吴祥红;金岳雷;;纳米材料的细胞毒性研究进展[J];中国优生与遗传杂志;2016年04期
2 徐雯;江俊康;杨北平;梅德清;戴兵;;不同粒径汽车尾气颗粒物对A549细胞毒性作用的比较[J];环境科学学报;2013年12期
3 李可欣;曹红英;王斌;张羽中;史科路;陶澍;;三种典型纳米颗粒物造成的人体肺细胞氧化应激和炎症效应[J];生态毒理学报;2010年06期
4 杨辉;杨丹凤;张华山;张伟;刘焕亮;刘超;袭著革;;4种典型纳米材料对小鼠胚胎成纤维细胞毒性的初步研究[J];生态毒理学报;2007年04期
相关博士学位论文 前1条
1 杜忠君;气管滴注纳米二氧化硅颗粒致大鼠心血管毒性及其相关机制研究[D];吉林大学;2013年
【共引文献】
相关期刊论文 前10条
1 孙悦;刘嘉，
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