镉、毒死蜱和量子点对肿瘤细胞增殖的影响研究
发布时间：2018-12-12 15:16
【摘要】：环境污染风险评估中混合物毒性是一个重要的问题,大量数据可为环境健康效应评估提供材料。然而,在现有的技术方面要解释复合物的相互作用还是很困难的。重金属和有机磷农药污染对人类有潜在的健康风险。尽管先前的研究证实镉(Cd)和毒死蜱(CPF)在环境介质和食物链中是共存的,但是它们之间的相互作用和潜在的复合毒性机制还很不清楚。本研究采用TLC和1H NMR来研究镉离子和毒死蜱的相互作用而诱导的肝脏毒性。本研究发现Cd2+和CPF之间可以发生一种新的相互作用,可能是Cd2+结合在CPF的吡啶环上,也有可能是一个Cd2+螯合两个CPF分子。镉与毒死蜱形成的复合物毒性明显高于它们单独时候的毒性,对毒死蜱与镉复合暴露后进行研究发现其胞内产生明显的氧化损伤效应。利用核磁共振技术探讨两类污染物间的相互关系,发现两者很有可能形成一种稳定的螯合物存在于环境中,并且比单独存在时有更强的毒性,对环境造成更严重的危害。 癌症是现代社会广泛关注的人类第二大高死亡率的疾病,现阶段常规手段的治疗主要有化疗放疗等生物化学手段,或者是手术方法。而这些治疗手段由于科学技术方面的限制,会对人类健康产生很多副作用。因此,新颖的抗癌疗法急需去改善癌症临床治疗并减少副作用。纳米科技和纳米材料的快速应用对于抗癌治疗中起重要作用。然而,怎么去把新的纳米技术和纳米材料应用到癌症的治疗中还有待研究。在本研究中,我们通过实验发现在宫颈癌细胞中量子点可以通过抑制ROCK的活性抑制ROCK-c-Myc信号通路从而抑制宫颈癌的发生发展。
[Abstract]:Toxicity of mixtures is an important problem in environmental pollution risk assessment, and a large amount of data can be used to assess environmental health effects. However, it is difficult to explain the interaction of the complex in existing techniques. Pollution of heavy metals and organophosphorus pesticides has potential health risks to human beings. Although previous studies have confirmed that cadmium (Cd) and chlorpyrifos (CPF) coexist in environmental media and food chain, the interaction between them and the underlying mechanism of compound toxicity are still unclear. In this study, TLC and 1H NMR were used to study the hepatotoxicity induced by the interaction of cadmium and chlorpyrifos. In this study, we found that a new interaction can occur between Cd2 and CPF. It may be that Cd2 binds to the pyridine ring of CPF or that one Cd2 chelates two CPF molecules. The toxicity of the complex formed by cadmium and chlorpyrifos was higher than that of chlorpyrifos alone. Nuclear magnetic resonance (NMR) was used to study the relationship between the two kinds of pollutants. It was found that a stable chelate exists in the environment, which is more toxic and more serious to the environment than when it exists alone. Cancer is the second highest mortality disease in modern society. At present, the conventional treatment mainly includes chemotherapy, radiotherapy and other biochemical methods, or surgical methods. These treatments, due to scientific and technological constraints, can have many side effects on human health. Therefore, novel anti-cancer therapies urgently need to improve the clinical treatment of cancer and reduce side effects. The rapid application of nanotechnology and nanomaterials plays an important role in anticancer therapy. However, how to apply new nanotechnology and nanomaterials to cancer treatment remains to be studied. In this study, we found that in cervical cancer cells, quantum dots can inhibit the development of cervical cancer by inhibiting the activity of ROCK and inhibiting the ROCK-c-Myc signaling pathway.
【学位授予单位】：山西大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114
[Abstract]:Toxicity of mixtures is an important problem in environmental pollution risk assessment, and a large amount of data can be used to assess environmental health effects. However, it is difficult to explain the interaction of the complex in existing techniques. Pollution of heavy metals and organophosphorus pesticides has potential health risks to human beings. Although previous studies have confirmed that cadmium (Cd) and chlorpyrifos (CPF) coexist in environmental media and food chain, the interaction between them and the underlying mechanism of compound toxicity are still unclear. In this study, TLC and 1H NMR were used to study the hepatotoxicity induced by the interaction of cadmium and chlorpyrifos. In this study, we found that a new interaction can occur between Cd2 and CPF. It may be that Cd2 binds to the pyridine ring of CPF or that one Cd2 chelates two CPF molecules. The toxicity of the complex formed by cadmium and chlorpyrifos was higher than that of chlorpyrifos alone. Nuclear magnetic resonance (NMR) was used to study the relationship between the two kinds of pollutants. It was found that a stable chelate exists in the environment, which is more toxic and more serious to the environment than when it exists alone. Cancer is the second highest mortality disease in modern society. At present, the conventional treatment mainly includes chemotherapy, radiotherapy and other biochemical methods, or surgical methods. These treatments, due to scientific and technological constraints, can have many side effects on human health. Therefore, novel anti-cancer therapies urgently need to improve the clinical treatment of cancer and reduce side effects. The rapid application of nanotechnology and nanomaterials plays an important role in anticancer therapy. However, how to apply new nanotechnology and nanomaterials to cancer treatment remains to be studied. In this study, we found that in cervical cancer cells, quantum dots can inhibit the development of cervical cancer by inhibiting the activity of ROCK and inhibiting the ROCK-c-Myc signaling pathway.
【学位授予单位】：山西大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114
【共引文献】
相关期刊论文 前3条
1 ;Spatial and temporal distribution of acetochlor in sediments and riparian soils of the Songhua River Basin in northeastern China[J];Journal of Environmental Sciences;2011年10期
2 曹s，
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