温室作业人员农药暴露致外周血淋巴细胞基因组不稳定表型

发布时间：2018-12-30 11:16

【摘要】：[目的]分析温室作业农药暴露对菜农外周血淋巴细胞基因组不稳定性的影响及其细胞毒作用。[方法]对安丘市从事温室蔬菜大棚种植者583名及普通农民576名进行问卷调查,根据农药暴露强度,将蔬菜大棚种植组所有调查对象分为低(n=175)、中(n=181)、高(n=212)3个暴露组。利用胞质分裂阻滞法微核细胞组学试验评价温室农药暴露所致菜农基因组不稳定性和细胞毒性。[结果]随着农药暴露水平的增加,微核率、核质桥、核芽发生率、细胞坏死率均有增加趋势(均P0.01),双核细胞率和核分裂指数具有降低趋势(P0.01),而凋亡细胞未发现明显的变化趋势。与对照组比较,中、高暴露组上述各指标差异皆具有统计学意义(均P0.05)。[结论]温室作业人群农药暴露会诱发基因组不稳定表型,并具有较强的细胞毒性。
[Abstract]:[objective] to analyze the effect of pesticide exposure in greenhouse on genomic instability of peripheral blood lymphocytes of vegetable farmers and its cytotoxicity. [methods] A questionnaire survey was conducted among 583 greenhouse growers and 576 ordinary farmers in Anqiu city. According to the pesticide exposure intensity, all the subjects in the vegetable greenhouse planting group were divided into low (nl175) and middle (nhl181) groups. There were 3 exposure groups with high (n = 212) exposure. The genomic instability and cytotoxicity of vegetable growers induced by exposure to greenhouse pesticides were evaluated by cytokinesis block assay. [results] with the increase of pesticide exposure level, the rate of micronucleus, nuclear cytoplasmic bridge, nuclear bud, cell necrosis rate increased (P0.01), and the rate of binuclear cells and mitotic index decreased (P0.01). However, no obvious change trend was found in apoptotic cells. Compared with the control group, the differences of the above indexes in the middle and high exposure groups were statistically significant (P0.05). [conclusion] Pesticide exposure can induce genomic instability phenotype and has strong cytotoxicity in greenhouse exposed population.
【作者单位】：潍坊医学院公共卫生与管理学院;
【基金】：山东省自然科学基金(编号:ZR2013HQ015) 潍坊医学院科技创新基金(编号:K11QC1004) 潍坊医学院“健康山东”重大社会风险预测与治理协同创新中心重点研究方向(编号:XT1406002)
【分类号】：R139

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