不同粒径纳米硫化铅对大鼠睾丸组织及精子生成的影响

发布时间：2018-12-29 19:56

【摘要】：目的探讨不同粒径的纳米硫化铅对雄性大鼠生殖能力的影响。方法将24只清洁级SD雄性大鼠随机分为4组,分为对照(生理盐水)组和15、35、75 nm纳米硫化铅染毒组,每组6只。采用灌胃方式进行染毒,染毒用量为10 ml/kg,每天1次,连续染毒8周。测定大鼠的精子存活率,并对睾丸进行病理学观察。结果与对照组相比,不同粒径纳米硫化铅染毒组大鼠的精子存活率均较低,差异有统计学意义(P0.05);且随着纳米硫化铅粒径的增大,大鼠的精子存活率呈上升趋势。病理学观察结果显示,75 nm纳米硫化铅染毒组生精小管变化不明显,各级精母细胞略显减少;35 nm纳米硫化铅染毒组生精小管内各级精母细胞数量减少;15 nm纳米硫化铅染毒组生精小管萎缩呈空网状,管腔内生精细胞脱落坏死。结论纳米硫化铅可损伤大鼠睾丸组织,影响精子生成,具有雄性生殖毒性,且粒径越小毒性越大。
[Abstract]:Objective to study the effect of nanometer lead sulfide with different particle size on reproductive ability of male rats. Methods Twenty-four clean grade SD male rats were randomly divided into 4 groups: control group (normal saline) group and 1535 nm nanometer lead sulfide group (n = 6 in each group). The dosage was 10 ml/kg, once a day for 8 weeks. The survival rate of spermatozoa and the pathological observation of testis were measured. Results compared with the control group, the spermatozoa survival rate of the rats exposed to lead sulphide nanoparticles with different particle sizes was lower (P0.05), and the sperm survival rate of the rats increased with the increase of the particle size of lead sulfide nanoparticles (P05). The pathological observation showed that the seminiferous tubules in 75 nm nano-lead sulfide group had no obvious changes, and the number of spermatocytes in the seminiferous tubules of 35 nm nano-lead sulfide group decreased slightly, and the number of seminiferous tubules in 35 nm nano-lead sulfide group was lower than that in the control group. The seminiferous tubules in 15 nm lead sulfide group showed hollow reticular atrophy, and spermatogenic cells in the tube were exfoliated and necrotized. Conclusion Nano-lead sulfide can damage testis of rats and affect spermatogenesis. It has male reproductive toxicity, and the smaller the particle size, the greater the toxicity.
【作者单位】：华北理工大学公共卫生学院河北省煤矿安全与卫生实验室;
【基金】：唐山市科技计划项目(14130231B) 华北理工大学青年基金(Z201425)
【分类号】：R114

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