沙棘粕醇提取物对秀丽隐杆线虫的抗衰老功效及其机制

发布时间：2018-12-30 16:13

【摘要】：以沙棘粕为研究对象,经乙醇提取、大孔吸附树脂纯化获得沙棘粕醇提取物(sea buckthorn seed extracts,SBSE)。以秀丽隐杆线虫为模式生物,研究SBSE的抗衰老作用及其可能机制。本研究考察了不同质量浓度范围内SBSE对秀丽隐杆线虫生命周期、生殖能力和应激抵御能力的影响,探索了SBSE对线虫抗氧化酶活力以及衰老相关基因表达水平的作用,从基因水平探索SBSE的抗衰老可能机制。结果:SBSE可以显著延长秀丽隐杆线虫的存活时间(P0.05),减轻脂褐素水平的积累;0.05 mg/mL SBSE处理后秀丽隐杆线虫子代数量为(246.40±21.11)个,显著高于空白对照组的(205.80±20.42)个(P0.05);对热应激和氧化应激有显著的防御效果(P0.05);此外,SBSE提高了秀丽隐杆线虫体内超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活力,降低了秀丽隐杆线虫促衰老基因age-1、daf-2的表达水平,提高了抗衰老基因sir-2.1的表达。该结果表明SBSE或许通过提高抗氧化酶活力以及改变衰老相关基因的表达水平来发挥抗衰老的功效。
[Abstract]:Hippophae rhamnoides meal was extracted by ethanol and purified by macroporous adsorption resin. The alcohol extract of Hippophae rhamnoides meal (sea buckthorn seed extracts,SBSE) was obtained. The antiaging effect of SBSE and its possible mechanism were studied with the model organism of Stereus elegans. In this study, we investigated the effects of SBSE on the life cycle, reproductive ability and stress resistance of nematodes in different concentrations, and explored the effects of SBSE on the activity of antioxidant enzymes and the expression level of senescence related genes in nematodes. To explore the possible anti-aging mechanism of SBSE at the gene level. Results: SBSE could significantly prolong the survival time (P0.05) and reduce the accumulation of lipofuscin. There were (246.40 卤21.11) and (205.80 卤20.42) worms in the control group after mg/mL SBSE treatment, which had a significant defense effect against heat stress and oxidative stress (P0.05). The number of insect generations was (246.40 卤21.11), significantly higher than that of the control group (205.80 卤20.42) (P0.05), and there were significant defense effects against heat stress and oxidative stress (P0.05). In addition, SBSE increased the activities of superoxide dismutase, catalase and glutathione peroxidase, and decreased the expression level of senescence gene age-1,daf-2. The expression of anti-aging gene sir-2.1 was increased. The results suggest that SBSE may play a role in anti-aging by increasing the activity of antioxidant enzymes and changing the expression level of senescence related genes.
【作者单位】：北京工商大学理学院植物资源研究开发北京市重点实验室;北京工商大学北京食品营养与人类健康高精尖创新中心;北京工商大学食品学院食品添加剂与配料工程研究中心;
【基金】：国家自然科学基金面上项目(31571801)
【分类号】：R151

【相似文献】