PAS-Na对染锰大鼠血清脂质过氧化、血和骨骼金属元素水平的影响

发布时间：2019-01-03 14:39

【摘要】：目的探讨对氨基水杨酸钠(PAS-Na)对染锰大鼠血清脂质过氧化、血和骨骼金属元素水平的影响。方法28只SD雄性大鼠被随机分为对照组、染锰组、低、高剂量PAS-Na治疗(L-、H-PAS)组。染锰组、L、H-PAS组腹腔注射(ip)Mn Cl2·4H2O 15 mg/kg,对照组ip等容量生理盐水,每日1次,每周5 d,连续3周。然后,L、H-PAS组背部皮下注射(sc)PAS-Na 100和200 mg/kg,对照组和染锰组背部sc等容量生理盐水,每日1次,连续5周。测定血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量,测定血、股骨、髂骨锰(Mn)、铁(Fe)、锌(Zn)、铜(Cu)、钙(Ca)和镁(Mg)含量。结果与对照组比较,染锰组血清MDA含量增高,H-PAS组MDA含量降低(P0.05)。血、股骨、髂骨Mn含量比对照组高,L-PAS组比对照组低(P0.05);染锰组血Fe、Mg含量均高于对照组,Zn含量低于对照组,L-PAS组Mg含量降低(P0.05);染锰组髂骨Cu、Mg含量均低于对照组(P0.05)。结论过量锰暴露可致机体产生脂质过氧化,干扰Fe、Cu、Mg、Zn含量的分布。PAS-Na有促排锰作用,对锰致血脂质过氧化和镁含量影响有干预作用。
[Abstract]:Objective to investigate the effects of sodium p-aminosalicylate (PAS-Na) on serum lipid peroxidation, blood and bone metal elements levels in manganese exposed rats. Methods 28 male SD rats were randomly divided into two groups: control group, low dose and high dose PAS-Na group. In manganese exposed group, (ip) Mn Cl2 4H2O 15 mg/kg, control group was intraperitoneally injected with normal saline of the same volume, 5 days a week for 3 weeks. Then, (sc) PAS-Na 100 and 200 mg/kg, sc were injected subcutaneously into the back of LHPAS group and manganese exposed group. Sc was given once a day for 5 weeks. Serum superoxide dismutase (SOD),) glutathione peroxidase (GSH-Px) activity, malondialdehyde (MDA) content, blood, femur, manganese, iron, (Fe), zinc, (Zn), (Cu), were measured. Calcium (Ca) and magnesium (Mg) contents. Results compared with the control group, the level of serum MDA in manganese exposed group was higher than that in control group, while the MDA content in H-PAS group was lower than that in control group (P0.05). Blood, femur, iliac bone Mn content was higher than the control group, L-PAS group was lower than the control group (P0.05), manganese group blood Fe,Mg content was higher than control group, Zn content was lower than control group, L-PAS group Mg content decreased (P0.05). The Cu,Mg content of iliac bone in manganese exposed group was lower than that in control group (P0.05). Conclusion excessive manganese exposure can induce lipid peroxidation and interfere with the distribution of Fe,Cu,Mg,Zn content. PAS-Na can promote manganese excretion and interfere with manganese induced lipid peroxidation and magnesium content in blood.
【作者单位】：广西医科大学公共卫生学院卫生毒理学教研室;南宁市疾病预防控制中心;
【基金】：国家自然科学基金(81072320,81460505) 广西自然科学基金(2014GXNSFAA118232)
【分类号】：R114

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