发酵法分离玉米皮水解液中L-阿拉伯糖的研究

发布时间：2019-01-05 03:43

【摘要】：L-阿拉伯糖是一种新兴的功能性单糖,具有控制血糖升高、抑制脂肪生成、促进双歧杆菌生长等作用,如何快速高效的分离提取出高纯度的L-阿拉伯糖已经成为研究的热点问题。目前工业上生产L-阿拉伯糖大多使用酸解半纤维素的方法,产物为多种单糖混合物。本实验针对目前树脂或薄层色谱分离L-阿拉伯糖操作复杂且收效小的缺点,对L-阿拉伯糖的微生物分离方法进行研究,论文考察了不同种类微生物对玉米皮水解液中三种主要单糖,即葡萄糖、木糖和L-阿拉伯糖的利用情况,并取得了一定的成果。文章首先对原材料和初提方法做了一个简单的筛选,确定使用硫酸水解玉米皮制备出玉米皮酸水解液。利用高效液相色谱法确定了其中主要单糖的含量,葡萄糖、木糖和L-阿拉伯糖的含量分别占玉米皮干物质的6.11%、18.82%和11.34%。并依此制备了模拟培养基。之后通过对常见的5种酵母菌、7种霉菌、7种细菌、4种乳酸菌和两株自坏境中分离筛选的霉菌Mgb1、Mgb2进行模拟培养基的发酵,并使用高效液相色谱法对发酵期间的发酵液进行检测。结果表明所有菌株在1-2d内都可将葡萄糖完全利用,细菌和乳酸菌不适用于对木糖和L-阿拉伯糖的分离。酵母菌中的球拟酵母,霉菌中的绿木霉、灰霉、Mgb1和Mgb2,对木糖和L-阿拉伯糖的利用具有较好的选择性,在发酵3d之后木糖能够被消耗掉绝大部分,剩余率仅为5.8%、2%、5.2%、4.5%和3.9%。而L-阿拉伯糖还有大量的保留,保留率为83.4%、70%、76.8%、88.6%和77.7%。将模拟培养后筛选出的球拟酵母、绿木霉、灰霉、Mgb1、Mgb2五种菌株用于对玉米皮酸水解液的发酵并使用高效液相色谱法对发酵期间的发酵液进行检测。结果表明,球拟酵母发酵4d,其余4种菌株发酵3d以后木糖的剩余量均小于5%,而L-阿拉伯糖的保留率可达75%以上。分离方法简单有效,所用的球拟酵母、绿木霉、灰霉3种菌株常见易得,为微生物法分离玉米皮水解液中的L-阿拉伯糖提供了一定的参考。
[Abstract]:L-arabinose is a new functional monosaccharide, which can control the increase of blood sugar, inhibit fat production and promote the growth of Bifidobacterium. How to extract high purity L-arabinose quickly and efficiently has become a hot issue. At present, L-arabinose is produced by acidolysis of hemicellulose. In this experiment, the microbiological separation method of L- arabinose was studied in allusion to the disadvantages of complex operation and low efficiency in the separation of L-arabinose by resin or thin-layer chromatography. In this paper, the utilization of three main monosaccharides, glucose, xylose and L-arabinose, in corn husk hydrolysate by different kinds of microorganisms was investigated, and some results were obtained. In this paper, a simple screening of raw materials and initial extraction methods was carried out, and the acid hydrolysate of corn husk was prepared by sulfuric acid hydrolysis. The main monosaccharides were determined by HPLC, and the contents of glucose, xylose and L-arabinose accounted for 6.1118.82% and 11.34% of the dry matter of corn skin, respectively. Based on this, the simulated medium was prepared. Then the fermentation medium was used to simulate the fermentation of 5 kinds of yeast, 7 kinds of mold, 7 kinds of bacteria, 4 kinds of lactic acid bacteria and 2 strains of fungi isolated from the bad environment. The fermentation broth during fermentation was detected by high performance liquid chromatography (HPLC). The results showed that all strains could make full use of glucose within 1-2 days, and bacteria and lactic acid bacteria were not suitable for the separation of xylose and L-arabinose. The utilization of xylose and L-arabinose by Mgb1 and Mgb2, was highly selective. After three days of fermentation, most of the xylose could be consumed, and the residual rate was only 5.8%. 2.5.2% and 3.9%. However, there are still a lot of reservations for L-arabinose, with a retention rate of 83.4% and 77.7%, 77.6% and 77.7%, respectively. Five strains, Trichoderma vulgaris, Trichoderma viride, Aspergillus graminearum and Mgb1,Mgb2, which were selected after simulated culture, were used to ferment the hydrolysate of corn peel acid and the fermentation broth during fermentation was detected by high performance liquid chromatography (HPLC). The results showed that the residual amount of xylose of the other four strains was less than 5 after 3 days fermentation, and the retention rate of L-arabinose could reach more than 75%. The isolation method is simple and effective, and the three strains of Trichoderma viride, Trichoderma viride and Trichoderma graminearum are common and easy to obtain, which provides a certain reference for the microbial separation of L-arabinose from corn husk hydrolysate.
【学位授予单位】：天津科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R151.2

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