益生菌发酵豆乳对高脂血症小鼠降血脂及肝损伤缓解的作用

发布时间：2019-01-25 21:24

【摘要】：研究干酪乳杆菌、植物乳杆菌和双歧杆菌在体外降胆固醇能力及三种益生菌混合发酵豆乳对高脂饮食小鼠血脂及肝损伤的影响。采用邻苯二甲醛法评价益生菌体外降胆固醇能力。采用C57BL/6N小鼠,并将其随机分为7组,即对照组;高脂组;干酪乳杆菌发酵豆乳干预组;植物乳杆菌发酵豆乳干预组;双歧杆菌发酵豆乳干预组;三种益生菌混合发酵豆乳干预组;未发酵豆乳干预组。连续喂养7周后,测定血清和肝脏中相关血脂指标,并观察肝脏组织病理学变化。结果表明:干酪乳杆菌、植物乳杆菌和双歧杆菌在体外降胆固醇能力分别为43.14%、46.27%和40.53%。益生菌混合发酵豆乳显著降低小鼠腹部和肾周脂肪指数,血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL),升高高密度脂蛋白(HDL)水平,降低肝脏TC、TG、丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平,并明显改善肝脏脂肪堆积。
[Abstract]:The effects of Lactobacillus casei Lactobacillus plantarum and Bifidobacterium on cholesterol lowering in vitro and the effects of three probiotics mixed fermented soybean milk on blood lipids and liver injury in mice with high fat diet were studied. The ability of probiotics to reduce cholesterol in vitro was evaluated by phthalaldehyde method. C57BL/6N mice were randomly divided into 7 groups: control group; high-fat group; Lactobacillus casei fermented soybean milk intervention group; Lactobacillus plantarum fermented soybean milk intervention group; bifidobacterium fermented soybean milk intervention group; Three kinds of probiotics mixed fermented soybean milk intervention group; unfermented soybean milk intervention group. After 7 weeks of continuous feeding, serum and liver blood lipid levels were measured and liver histopathological changes were observed. The results showed that the cholesterol lowering ability of Lactobacillus casei, Lactobacillus plantarum and Bifidobacterium in vitro was 46.27% and 40.53%, respectively. Probiotic mixed fermented soybean milk significantly decreased abdominal and perirenal fat index, serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), increased the level of high density lipoprotein (HDL) and decreased liver TC,TG,. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly improved.
【作者单位】：吉林农业大学食品科学与工程学院;国家大豆产业技术体系加工研究室;
【基金】：科技部“十二五”科技支撑计划项目(2013BAD18B07) 吉林省教育厅吉林省高校新世纪科学技术优秀人才项目(201439)
【分类号】：R151

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