氯化甲基汞对秀丽隐杆线虫运动和感觉功能的影响

发布时间：2019-01-26 16:38

【摘要】：目的分析神经毒物氯化甲基汞对模式生物秀丽隐杆线虫运动和感觉行为的影响,初步探讨利用秀丽隐杆线虫研究氯化甲基汞神经毒性的可能性。方法采用氯化甲基汞对L1期、L4期幼虫分别急性(30 min)染毒[终浓度分别为0(溶剂对照)~202.5μmol/L和0~1 200μmol/L]和慢性(24 h)染毒[终浓度分别为0(溶剂对照)~80μmol/L和0~150μmol/L]。测定半数致死浓度(LC50)及行为学指标。结果氯化甲基汞致L1期、L4期秀丽隐杆线虫的LC50(30 min)分别为(65.93±8.89)μmol/L和(206.71±35.43)μmol/L,LC50(24 h)分别为(11.59±0.79)μmol/L和(24.89±4.88)μmol/L,L1期秀丽隐杆线虫对氯化甲基汞的毒性较L4期敏感。随着氯化甲基汞暴露剂量的升高,急性和慢性染毒L1期、L4期秀丽隐杆线虫的弯曲频率和头部摆动频率均呈下降的趋势,基本运动能力呈减弱趋势,触碰反应呈迟钝的趋势。结论氯化甲基汞可抑制运动和感觉功能,秀丽隐杆线虫可用于氯化甲基汞神经毒性的研究。
[Abstract]:Objective to analyze the effects of methylmercury chloride on the motility and sensory behavior of the model organism, and to explore the possibility of studying the neurotoxicity of methylmercury chloride by using the nematode. Methods methylmercury chloride was used to treat phase L1, L4 larvae were exposed to acute (30 min) (final concentration 0 (solvent control) ~ 202.5 渭 mol/L and 0.200 渭 mol/L) and chronic (24 h) exposure to 0 (solvent control) ~ 80 渭 mol/L and 0 ~ (150 渭 mol/L) respectively. The median lethal concentration (LC50) and behavioral index were measured. Results the LC50 (30 min) of methylmercury chloride in stage L1 and stage L 4 were (65.93 卤8.89) 渭 mol/L and (206.71 卤35.43) 渭 mol/L, respectively. LC50 (24 h) was (11.59 卤0.79) 渭 mol/L and (24.89 卤4.88) 渭 mol/L,L1, respectively. With the increase of exposure dose of methylmercury chloride, the bending frequency and head swing frequency of C. elegans in acute and chronic phase L1 and L4 decreased, while the basic motor ability decreased, and the contact reaction showed a slow tendency. Conclusion methylmercuric chloride can inhibit motor and sensory function, and cryptospira can be used to study the neurotoxicity of methylmercury chloride.
【作者单位】：华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系环境与健康教育部重点实验室;华中科技大学同济医学院公共卫生学院营养与食品卫生学系;
【基金】：中央高校基本科研业务费专项资金资助项目(2013TS011) 国家高科技研究发展(863)计划(2013AA065204)
【分类号】：R114

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