慢性激光刺激对人体衰老相关指标的影响

发布时间：2019-01-27 14:47

【摘要】：目的了解慢性激光刺激对人体衰老相关指标的影响。方法将168名某激光研究所人员按是否进行激光作业分为暴露组(n=90)和非暴露组(n=78)。采用固相夹心法酶联免疫吸附测定(ELISA)检测血浆Klotho蛋白含量,采用比色法检测红细胞超氧化物歧化酶(SOD)活力及丙二醛(MDA)浓度,采用流式荧光原位杂交(Flow-FISH)检测淋巴细胞端粒长度。结果暴露组及非暴露组人员血浆Klotho蛋白水平、红细胞MDA浓度、淋巴细胞端粒长度的差异无统计学意义(P0.05);多元回归分析显示血浆Klotho蛋白、红细胞SOD与MDA、淋巴细胞端粒长度与激光暴露无明显相关,亚组分析显示暴露组中吸烟人员的红细胞SOD浓度明显低于非暴露组(P0.05)。结论慢性激光刺激对衰老相关指标无直接影响,但可能导致吸烟人员红细胞SOD浓度降低。
[Abstract]:Objective to investigate the effect of chronic laser stimulation on human aging. Methods 168 workers of a laser research institute were divided into exposure group (ng 90) and non-exposed group (n-78) according to whether they were exposed to laser or not. The content of plasma Klotho protein was determined by enzyme linked immunosorbent assay (Elisa) with solid phase sandwich method. The activity of superoxide dismutase (SOD) and malondialdehyde (MDA) in erythrocytes were detected by colorimetry. Telomere length of lymphocytes was measured by flow fluorescence in situ hybridization (Flow-FISH). Results there was no significant difference in plasma Klotho protein level, erythrocyte MDA concentration and telomere length between exposed group and non-exposed group (P0.05). Multivariate regression analysis showed that there was no significant correlation between plasma Klotho protein, telomere length of erythrocyte SOD and MDA, lymphocytes and laser exposure. Subgroup analysis showed that the erythrocyte SOD concentration in the exposed group was significantly lower than that in the non-exposed group (P0.05). Conclusion chronic laser stimulation has no direct effect on aging related indexes, but it may lead to the decrease of erythrocyte SOD concentration in smokers.
【作者单位】：第三军医大学西南医院老年科;
【基金】：国家自然科学基金资助项目(81000132)
【分类号】：R454.2

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