北京市售即食熟肉制品中单核细胞增生李斯特菌定量污染水平研究

发布时间：2019-02-09 19:15

【摘要】：目的了解北京市售即食熟肉制品中单核细胞增生李斯特菌污染情况及污染来源。方法采用MPN法定量检测即食熟肉制品中的单核细胞增生李斯特菌,在对分离菌株正确鉴定的基础上,对单核细胞增生李斯特菌分离株进行脉冲场凝胶电泳分型研究。结果 197份即食熟肉制品中有14份检出单核细胞增生李斯特菌,检出率为7.11%,平均污染水平为14.31 MPN/g。其中农贸市场采集的样品单核细胞增生李斯特菌污染率(13.89%)高于熟肉专卖店(8.57%)、超市(5.75%)和饭馆(2.56%)。而超市采集样品中该菌的平均污染水平最高,为22.78 MPN/g。共发现13种单核细胞增生李斯特菌PFGE型,其中从同一农贸市场采集的样品中分离到两株同一个PFGE型别的单核细胞增生李斯特菌,提示加工环境存在单核细胞增生李斯特菌的污染或加工过程的交叉污染。结论北京市售即食熟肉制品中存在单核细胞增生李斯特菌污染,农贸市场出售产品污染状况高于超市、熟肉专卖店和饭馆。
[Abstract]:Objective to investigate the contamination of Listeria monocytogenes in ready-to-eat cooked meat products in Beijing. Methods Listeria monocytogenes (Listeria monocytogenes) in ready-to-eat cooked meat products were detected by MPN assay. Based on the correct identification of the isolated strains, the isolation of Listeria monocytogenes was studied by pulsed field gel electrophoresis (PGE). Results Listeria monocytogenes were detected in 14 out of 197 ready-to-eat cooked meat products. The detection rate was 7.11 and the average contamination level was 14.31 MPN/g.. The contamination rate of Listeria monocytogenes collected from farmers' markets (13.89%) was higher than that of cooked meat shops (8.57%), supermarkets (5.75%) and restaurants (2.56%). The average contamination level of this bacterium was 22.78 MPN/g. in supermarket samples. Thirteen PFGE types of Listeria monocytogenes were found. Two strains of Listeria monocytogenes with the same PFGE type were isolated from the samples collected from the same farmers' market. The results suggest that there is contamination of Listeria monocytogenes or cross-contamination during processing in processing environment. Conclusion the contamination of Listeria monocytogenes in Beijing ready-to-eat cooked meat products is higher than that in supermarkets, cooked meat shops and restaurants.
【作者单位】：国家食品安全风险评估中心卫生部食品安全风险评估重点实验室;
【基金】：国家高技术研究发展计划(863计划)(No.2012AA10163)
【分类号】：R155.51

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