被动吸烟对雄性大鼠及子代学习记忆影响的研究

发布时间：2019-02-14 20:29

【摘要】：目的:模拟人群在吸烟环境中的暴露状态,构建不同剂量及不同时间吸烟的雄性大鼠被动吸烟模型,评估吸烟对雄性大鼠及子代学习记忆的影响。方法:选用SD大鼠150只(雄性100只,雌性50只),随机分为染毒组和空白对照组。雄鼠于处死前1周与雌鼠按1∶1合笼交配,雌鼠分娩后21天内观察仔鼠发育情况。测定雄性大鼠及子代脑组织中SOD活力、MDA及NO含量;HE染色观察脑、海马组织形态学改变;IHC法检测脑组织c-Fos及海马组织BDNF表达情况;RT-PCR及Western Blot法检测雄性大鼠及子代脑组织tau、海马组织NR2B基因mRNA及蛋白表达。结果:1.染毒4周组的中剂量和高剂量雄性大鼠体重随染毒剂量的增加而降低(P0.05)。2.各染毒组的雄性大鼠脑组织系数较对照组降低,差异有统计学意义(P0.05);各染毒组的仔鼠脑组织系数除4周组外,较对照组降低,差异有统计学意义(P0.05)。3.各染毒组的雄性大鼠脑SOD活力较对照组降低,差异有统计学意义(P0.05);各染毒组的仔鼠脑SOD活力较对照组降低,差异有统计学意义(P0.05)。4.各染毒组的雄性大鼠脑MDA含量较对照组上升,差异有统计学意义(P0.05);各染毒组的仔鼠脑MDA含量较对照组上升,差异有统计学意义(P0.05)。5.各染毒组的雄性大鼠脑NO含量除4周组外,较对照组上升,差异有统计学意义(P0.05);6周组的仔鼠脑NO含量,较对照组上升,差异有统计学意义(P0.05)。6.定位航行实验中,雄性大鼠潜伏期结果与对照组相比,差异有统计学意义(P0.05),游泳距离结果与对照组相比,差异有统计学意义(P0.05);仔鼠潜伏期结果与对照组相比,差异有统计学意义(P0.05),游泳距离结果与对照组相比,差异有统计学意义(P0.05)。7.空间探索实验中,雄性大鼠低、中、高剂量组中,不同染毒时间组比较,差异均有统计学意义(P0.05);仔鼠染毒4周组较对照组降低,差异有统计学意义(P0.05)。8.脑组织及海马组织HE染色观察发现,染毒组雄性大鼠脑组织及海马组织出现不同程度的损伤,并且随着染毒时间延长损伤更加明显;9.4周染毒组的雄性大鼠脑组织fos蛋白含量较对照组有所上升,差异有统计学意义(P0.05);各染毒组的仔鼠脑组织fos蛋白含量较对照组有所上升,差异有统计学意义(P0.05)。6、8周染毒组的雄性大鼠海马组织BDNF蛋白含量较对照组有所上升,差异有统计学意义(P0.05);4、6周染毒组的仔鼠海马组织BDNF蛋白含量较对照组有所上升,差异有统计学意义(P0.05)。10.与对照组相比,雄性大鼠6周中、高剂量组脑tau m RNA和蛋白表达水平增加明显(P0.05);8周中、高剂量染毒组脑tau mRNA和蛋白表达较对照组相比出现明显增加差异有统计学意义(P0.05);仔鼠染毒4周组高剂量,6周中、高剂量组,8周低剂量组脑组织tau蛋白表达水平较对照组升高,差异有统计学意义(P0.05)。与对照组相比,雄性大鼠6周染毒组组海马NR2B mRNA和蛋白表达水平降低明显(P0.05);8周染毒组海马NR2B mRNA和蛋白表达较对照组相比出现明显降低,差异有统计学意义(P0.05);仔鼠6、8周染毒组海马NR2B mRNA和蛋白表达较对照组相比出现明显降低,差异有统计学意义(P0.05)。结论:吸烟可导致雄性大鼠及子代行为学产生损伤改变,香烟暴露也可造成雄性大鼠及子代脑、海马组织的损伤,导致脑及海马组织蛋白及mRNA表达异常,提示吸烟可导致雄性大鼠及子代学习记忆的损伤。
[Abstract]:Objective: To study the effect of smoking on the learning and memory of male rats and children by simulating the exposure status of the population in the smoking environment, constructing the passive smoking model of the male rats with different doses and different time. Methods: 150 SD rats (100 males and 50 females) were randomly divided into exposure group and blank control group. Male rats were mated with female rats at 1: 1 in the first week before the sacrifice, and the development of the mice was observed in 21 days after the female. The activity of SOD, MDA and NO in the brain of the male and the offspring of the rats were measured. The morphological changes of the brain and the hippocampus were observed with HE staining. The expression of BDNF in the c-Fos and the hippocampus of the brain was detected by the IHC method, and the tau in the brain of the male and the offspring was detected by RT-PCR and Western Blot. mRNA and protein expression of NR2B gene in hippocampus. Results: 1. The weight of middle and high-dose male rats in the 4-week group decreased with the increase of the dose (P0.05). The brain tissue factor of the male rats in each group was lower than that in the control group (P <0.05). The brain tissue factor of the rats in each group was lower than that of the control group (P0.05). The activity of SOD in the brain of male rats in each group was lower than that in the control group (P <0.05). The activity of SOD in the brain of each group was lower than that in the control group (P <0.05). The content of MDA in the male rats of each group was higher than that in the control group (P <0.05). The content of MDA in the rats of each group was higher than that in the control group (P0.05). The NO content in the male rats of each group was higher than that in the control group except for the 4-week group (P0.05). The content of NO in the brain of the rats in the 6-week group was higher than that in the control group (P0.05). In the experiment of positioning, the latency of the male rats was significantly different from that of the control group (P0.05). The results of the swimming distance were statistically significant (P0.05). The results of swimming distance were statistically significant (P0.05). In the experiment of space exploration, in the low, middle and high dose group of male rats, the difference was significant (P0.05). The results showed that the level of fos protein in the brain and the hippocampus of the exposed group was higher than that in the control group. There was a significant difference in the content of fos protein in the hippocampus of each group (P0.05). The content of the expression of BDNF in the hippocampus of the rats in the 6 and 8-week group was higher than that in the control group (P <0.05). The content of BDNF protein in the hippocampus of the rats in 4 and 6 weeks was higher than that in the control group (P0.05). Compared with the control group, the level of tau m RNA and protein expression in the high-dose group was significantly higher than that in the control group (P0.05); in the 8-week period, the expression of tau mRNA and protein in the high-dose group was significantly higher than that of the control group (P0.05). The level of tau protein expression in the high-dose group and the high-dose group and the low-dose group in the 8-week low-dose group was significantly higher than that in the control group (P0.05). Compared with the control group, the expression of NR2B mRNA and protein in the hippocampus of the rats in the 6-week group was significantly lower than that in the control group (P <0.05). The expression of NR2B mRNA and protein in the hippocampus of the group was significantly lower than that of the control group (P0.05). The expression of NR2B mRNA and protein in the hippocampus of the rats in 6 and 8 weeks was significantly lower than that in the control group (P0.05). Conclusion: Smoking can cause damage to the behavior of male rats and offspring, and the exposure of cigarette can also cause the injury of the brain and hippocampus of the male and the offspring, which leads to the abnormal expression of the protein and the mRNA in the brain and the hippocampus. It is suggested that the smoking can lead to the learning and memory of the male and the offspring.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R114

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