锰暴露对PARK2表达的影响
发布时间：2019-02-27 15:26
【摘要】：目的：通过研究锰暴露对脑组织、血液、唾液中PARK2表达的影响,探寻锰暴露早期效应标志物。方法：实验分两部分进行。人群研究部分：选择锰铁合金厂三年以上工龄的炉前工人作为锰接触组(37名),以劳动强度相似的铁厂炉前工人作为对照组(41名)。原子吸收法(AAS)检测各组血液和唾液中锰浓度,实时定量反转录-聚合酶链反应法(RT-PCR)检测各组血液和唾液中PARK2的表达。动物实验部分：24只雄性SD大鼠(体重220g±10g)随机分为三组：对照组(Omg/kg Mn),低剂量组(1mg/kg Mn),高剂量组(5mg/kg Mn)。通过腹腔注射法染锰(对照组注射等量生理盐水),连续染锰四周,第四周末取大鼠脑组织(皮质、纹状体、海马、丘脑)。原子吸收法(AAS)检测各组脑组织中锰含量,实时定量反转录-聚合酶链反应法(RT-PCR)检测各组脑组织中PARK2表达,蛋白质免疫印迹法(WB)检测各组脑组织中Parkin蛋白含量。结果：人群研究结果显示：锰接触组工人血液和唾液中锰浓度明显高于对照组(P0.01)；与对照组比较,PARK2在血液和唾液中表达下降(P0.05)。动物实验结果显示：高剂量组和低剂量组大鼠(皮质、纹状体、海马、丘脑)中锰含量高于对照组(P0.05,P0.01)；与对照组比较,PARK2在高剂量组大鼠(皮质、纹状体、海马、丘脑)和低剂量组大鼠(纹状体、海马)中表达下降(P0.05),但是在低剂量组皮质、丘脑中表达差异无统计学意义(P0.05)；Parkin蛋白在高剂量组大鼠(皮质、纹状体、海马、丘脑)和低剂量组大鼠(纹状体、海马)中含量下降(P0.05,P0.01),但是在低剂量组大鼠皮质、丘脑中差异无统计学意义(P0.05)。结论：1.锰暴露可使脑组织(皮质、纹状体、海马、丘脑)、血液、唾液PARK2表达下降,提示可能是锰致神经毒性的机制之一；2.唾液口腔脱落细胞中PARK2的表达变化有作为锰暴露后早期效应标志物的可能。
[Abstract]:Objective: To study the effect of manganese exposure on the expression of PARK2 in brain, blood and saliva, and to explore the early effect marker of manganese exposure. Methods: The experiment was divided into two parts. The study part of the population: the former workers in the manganese-iron alloy factory for more than three years were selected as the manganese contact group (37), and the workers in the iron factory with similar labor intensity were used as the control group (41). The concentration of manganese in each group of blood and saliva was detected by AAS. The expression of PARK2 in blood and saliva was detected by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). Animal experiment part:24 male SD rats (weighing 220 g/10 g) were randomly divided into three groups: control group (Omg/ kg Mn), low-dose group (1 mg/ kg Mn) and high-dose group (5 mg/ kg Mn). The brain (cortex, striatum, hippocampus, thalamus) of the rat brain (cortex, striatum, hippocampus, thalamus) was obtained by intraperitoneal injection of manganese (the same amount of normal saline was injected into the control group). The expression of parkin in each group of brain tissue was detected by the method of atomic absorption (AAS), and the content of parkin in each group was detected by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: The results of the population study showed that the concentration of manganese in the blood and saliva of workers exposed to manganese was significantly higher than that in the control group (P0.01). In comparison with the control group, the expression of PARK2 in blood and saliva decreased (P0.05). The results of animal experiments showed that the content of manganese in high-dose group and low-dose group (cortex, striatum, hippocampus and thalamus) was higher than that in the control group (P0.05, P0.01); in comparison with the control group, PARK2 was in high-dose group (cortex, striatum, hippocampus, thalamus) and low-dose group (striatum, The expression of Parkin in the rat (cortex, striatum, hippocampus, thalamus) and low-dose group (striatum, hippocampus) of rats (cortex, striatum, hippocampus, thalamus) and low-dose group (P0.05, P0.01) was not significant (P0.05). However, there was no significant difference in the cortex and thalamus of the low-dose group (P0.05). Conclusion:1. Manganese exposure may decrease the expression of PARK2 in the brain (cortex, striatum, hippocampus, thalamus), blood, and saliva, suggesting that it may be one of the mechanisms of manganese-induced neurotoxicity;2. The expression of PARK2 in saliva-shedding cells has the potential to be a marker of early effect after exposure to manganese.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R135
本文编号：2431387
[Abstract]:Objective: To study the effect of manganese exposure on the expression of PARK2 in brain, blood and saliva, and to explore the early effect marker of manganese exposure. Methods: The experiment was divided into two parts. The study part of the population: the former workers in the manganese-iron alloy factory for more than three years were selected as the manganese contact group (37), and the workers in the iron factory with similar labor intensity were used as the control group (41). The concentration of manganese in each group of blood and saliva was detected by AAS. The expression of PARK2 in blood and saliva was detected by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). Animal experiment part:24 male SD rats (weighing 220 g/10 g) were randomly divided into three groups: control group (Omg/ kg Mn), low-dose group (1 mg/ kg Mn) and high-dose group (5 mg/ kg Mn). The brain (cortex, striatum, hippocampus, thalamus) of the rat brain (cortex, striatum, hippocampus, thalamus) was obtained by intraperitoneal injection of manganese (the same amount of normal saline was injected into the control group). The expression of parkin in each group of brain tissue was detected by the method of atomic absorption (AAS), and the content of parkin in each group was detected by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: The results of the population study showed that the concentration of manganese in the blood and saliva of workers exposed to manganese was significantly higher than that in the control group (P0.01). In comparison with the control group, the expression of PARK2 in blood and saliva decreased (P0.05). The results of animal experiments showed that the content of manganese in high-dose group and low-dose group (cortex, striatum, hippocampus and thalamus) was higher than that in the control group (P0.05, P0.01); in comparison with the control group, PARK2 was in high-dose group (cortex, striatum, hippocampus, thalamus) and low-dose group (striatum, The expression of Parkin in the rat (cortex, striatum, hippocampus, thalamus) and low-dose group (striatum, hippocampus) of rats (cortex, striatum, hippocampus, thalamus) and low-dose group (P0.05, P0.01) was not significant (P0.05). However, there was no significant difference in the cortex and thalamus of the low-dose group (P0.05). Conclusion:1. Manganese exposure may decrease the expression of PARK2 in the brain (cortex, striatum, hippocampus, thalamus), blood, and saliva, suggesting that it may be one of the mechanisms of manganese-induced neurotoxicity;2. The expression of PARK2 in saliva-shedding cells has the potential to be a marker of early effect after exposure to manganese.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R135
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