硒化合物在HepG2细胞中代谢生成硒蛋白效应

发布时间：2019-02-28 10:54

【摘要】：目的探讨亚硒酸钠(Na2Se O3),硒代蛋氨酸(Se Met)和甲基硒代半胱氨酸(Me Se Cys)3种硒化合物在Hep G2细胞内代谢生成硒蛋白P(SEPP)和谷胱甘肽过氧化物酶(GPx)效应。方法将Hep G2细胞分为对照组、低、中、高剂量硒化合物组(0.01、0.10、1.00μmol/L),作用48、72 h,收集细胞上清液和裂解液;采用双抗夹心酶联免疫吸附试验检测上清中SEPP浓度与裂解液中GPx活力。结果与对照组比较,高剂量Na2Se O3、Se Met、Me Se Cys作用Hep G2细胞48 h时,上清中SEPP浓度[分别为(115.78±1.70)、(140.73±0.97)、(114.92±1.46)pg/m L]均明显升高(P0.01),裂解液中GPx活力[分别为(490.52±5.40)、(643.26±5.40)、(603.35±6.40)U/L]明显升高(P0.01);与Na2Se O3、Me Se Cys比较,Se Met作用效果最好(P0.05);3种硒化合物作用Hep G2细胞48 h与72 h比较,各指标差异均无统计学意义。结论 3种硒化合物中,以Se Met作用于Hep G2细胞后代谢生成SEPP和GPx的效果最明显。
[Abstract]:Aim to investigate the effects of sodium selenite (Na2Se O 3), selenium methionine (Se Met) and methyl selenocysteine (Me Se Cys) on the metabolism of selenium protein P (SEPP) and glutathione peroxidase (GPx) in Hep G2 cells. Methods Hep G2 cells were divided into control group, low, middle and high dose selenium group (0.01 渭 mol / L), 0.10 渭 mol / L), 1.00 渭 mol / L), for 48 h, 72 h) to collect the supernatant and lysate. The concentration of SEPP in supernatant and the activity of GPx in lysate were measured by double antibody sandwich enzyme-linked immunosorbent assay (Elisa). Results compared with the control group, the concentration of SEPP in the supernatant of Hep G2 cells treated with high dose of Na2Se O 3 and se Met,Me Se Cys for 48 h [(115.78 卤1.70), (140.73 卤0.97), respectively], (114.92 卤1.46) pg/m / L and (490.52 卤5.40), (/ L (643.26 卤5.40), (603.35 卤6.40) U / L, respectively, significantly increased in the lysate (P0.01), and significantly increased in the lysate [(490.52 卤5.40), (643.26 卤5.40), (603.35 卤6.40) U / L]. Compared with Na2Se O3 and me Se Cys, Se Met had the best effect (P0.05), and there was no significant difference between the three selenium compounds for 48 h and 72 h in Hep G2 cells. Conclusion among the three selenium compounds, the effect of Se Met on the metabolism of Hep G2 cells to SEPP and GPx is the most obvious.
【作者单位】：中国疾病预防控制中心营养与健康所营养与代谢研究室;
【基金】：国家自然科学基金(81372989) 国家自然科学青年基金(31201352) 中国营养学会伊利营养与健康研究基金(2013-013)
【分类号】：R151

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