铝致大鼠认知能力和全基因组甲基化改变的研究

发布时间：2019-03-01 12:03

【摘要】：目的研究铝对大鼠认知能力及全基因组甲基化的影响。方法健康清洁级SD雄性大鼠72只,按体质量随机分成两部分9组,实验部分:对照组(生理盐水),低、中、高铝剂量组(0.27、0.54和1.08 mg/kg);干预部分:对照组(生理盐水),铝高剂量组(1.08 mg/kg),铝高剂量+叶酸低、中、高剂量组(0.7、1.5和3.4 mg/kg),每组8只。各组大鼠腹腔注射相应剂量麦芽酚铝(0.2 ml/d),同时叶酸各剂量干预组每日灌胃叶酸1 ml/100 g,连续60 d。采用Morris水迷宫测定大鼠学习记忆能力,采用ELISA法测定大鼠全基因组甲基化。结果 Morris水迷宫结果显示,铝暴露可导致大鼠目标象限停留时间明显缩短,穿越原平台位置的次数明显减少,铝低、中、高剂量组与对照组比较差异有统计学意义(P0.05);叶酸干预铝高剂量组可导致大鼠目标象限停留时间明显延长和穿越原平台位置的次数明显增多,铝高剂量组与铝高+叶酸中、高剂量组比较差异有统计学意义(P0.05)。大鼠全基因组甲基化测定结果显示:随铝染毒剂量的增加,0.54、1.08 mg/kg染毒组大鼠全基因组甲基化率显著降低,与对照组比较差异具有统计学意义(P0.05);而添加高剂量叶酸组全基因组甲基化率显著增加,与铝高剂量组比较差异具有统计学意义(P0.05),与对照组比较差异无统计学意义。结论铝可导致大鼠学习记忆能力下降,大鼠全基因组甲基化率降低,叶酸可能对其有改善作用。
[Abstract]:Objective to study the effects of aluminum on cognitive ability and whole genome methylation in rats. Methods 72 healthy and clean grade male SD rats were randomly divided into 9 groups according to body mass. The experimental part was as follows: control group (normal saline), low, middle, high aluminum dose group (0.27,0.54 and 1.08 mg/kg); Intervention part: control group (saline), high-dose aluminum group (1.08 mg/kg), high-dose aluminum folic acid low, middle, high-dose group (0.7, 1.5 and 3.4 mg/kg), 8 rats in each group. Rats in each group were intraperitoneally injected with the corresponding dose of maltol aluminum (0. 2 ml/d), and folic acid in the intervention group was given 1 ml/100 / g daily for 60 days. The learning and memory ability of rats was measured by Morris water maze, and the whole genome methylation of rats was determined by ELISA method. Results the results of Morris water maze showed that aluminum exposure could significantly shorten the retention time of the target quadrant, decrease the number of traversing the original platform position, and lower aluminum. There was significant difference between the middle and high dose groups compared with the control group (P0.05). The intervention of folic acid in the high-dose aluminum group could significantly prolong the target quadrant residence time and increase the times of crossing the original platform position. There was a significant difference between the high-dose aluminum group and the high-dose aluminum-folic acid group (P0.05). The results of whole genome methylation assay showed that the methylation rate of the whole genome in 0.54 and 1.08 mg/kg groups was significantly lower than that in the control group (P0.05). The whole genome methylation rate of the high-dose folic acid group was significantly higher than that of the high-dose aluminum group (P 0.05), but no significant difference was found between the high-dose folic acid group and the control group. Conclusion Aluminum can decrease the ability of learning and memory in rats and the methylation rate of whole genome in rats. Folic acid may improve the ability of learning and memory in rats.
【作者单位】：山西医科大学公共卫生学院;山西医科大学第一临床医学院老年病科;
【基金】：国家自然科学基金(No.81372968)
【分类号】：R114

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