亚砷酸钠对Hacat细胞GSK-3β及CyclinD1表达的影响
发布时间：2019-03-04 21:00
【摘要】：目的探讨亚砷酸钠对人上皮角质形成细胞系(Hacat细胞)PI3K/AKT信号通路中细胞周期相关分子GSK-3β、CyclinD1的影响。方法将人Hacat细胞暴露于浓度分别为0、10、25、50、100μmol/L的亚砷酸钠6 h,采用Western Blot法检测细胞总蛋白的GSK-3β,磷酸化GSK-3β(p-GSK-3β),CyclinD1和磷酸化CyclinD1(p-CyclinD1)的蛋白表达水平。结果与对照组比较,10、25、50、100μmol/L染砷组总蛋白中p-GSK-3β和CyclinD1蛋白表达水平均显著增高(P0.05),而p-CyclinD1蛋白表达水平显著降低(P0.05)。结论亚砷酸钠可影响Hacat细胞中的GSK-3β分子,使其磷酸化失活,进而失去对细胞周期相关蛋白CyclinD1的磷酸化作用,从而促进CyclinD1的表达。
[Abstract]:Aim to investigate the effect of sodium arsenite on GSK-3 尾 and CyclinD1 in PI3K/AKT signaling pathway of human epithelial keratinocytes (Hacat cells). Methods Human Hacat cells were exposed to sodium arsenite at concentrations of 0, 10, 25 and 50 100 渭 mol / L for 6 h, respectively. The expression levels of GSK-3 尾, phosphorylated GSK-3 尾 (p-GSK-3 尾), CyclinD1 and phosphorylated CyclinD1 (p-CyclinD1) were detected by Western Blot assay. Results compared with the control group, the expression levels of p-GSK-3 尾 and CyclinD1 protein in the total protein of 10,25,50100 渭 mol / L arsenic exposed group were significantly increased (P0.05), while the expression level of p-CyclinD1 protein was significantly decreased (P0.05). Conclusion Sodium arsenite can affect the GSK-3 尾 molecule in Hacat cells, make it phosphorylated and inactivated, and then lose the phosphorylation of CyclinD1, thus promote the expression of CyclinD1.
【作者单位】： 中国医科大学公共卫生学院劳动卫生教研室 "砷生物学作用评价及砷中毒防治"辽宁省重点实验室;
【基金】：辽宁省教育厅课题(编号L2010705)
【分类号】：R114
[Abstract]:Aim to investigate the effect of sodium arsenite on GSK-3 尾 and CyclinD1 in PI3K/AKT signaling pathway of human epithelial keratinocytes (Hacat cells). Methods Human Hacat cells were exposed to sodium arsenite at concentrations of 0, 10, 25 and 50 100 渭 mol / L for 6 h, respectively. The expression levels of GSK-3 尾, phosphorylated GSK-3 尾 (p-GSK-3 尾), CyclinD1 and phosphorylated CyclinD1 (p-CyclinD1) were detected by Western Blot assay. Results compared with the control group, the expression levels of p-GSK-3 尾 and CyclinD1 protein in the total protein of 10,25,50100 渭 mol / L arsenic exposed group were significantly increased (P0.05), while the expression level of p-CyclinD1 protein was significantly decreased (P0.05). Conclusion Sodium arsenite can affect the GSK-3 尾 molecule in Hacat cells, make it phosphorylated and inactivated, and then lose the phosphorylation of CyclinD1, thus promote the expression of CyclinD1.
【作者单位】： 中国医科大学公共卫生学院劳动卫生教研室 "砷生物学作用评价及砷中毒防治"辽宁省重点实验室;
【基金】：辽宁省教育厅课题(编号L2010705)
【分类号】：R114
【共引文献】
相关期刊论文 前10条
1 黄秀兰;崔国辉;周克元;;PI3K-Akt信号通路与肿瘤细胞凋亡关系的研究进展[J];癌症;2008年03期
2 孙嫣;田华;肖法Z，
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