花生粕发酵物对动物的延缓衰老及改善腹泻效果研究
发布时间：2019-03-16 23:00
【摘要】：本试验以花生粕为主要原料,利用纳豆芽孢杆菌固态发酵花生粕,探究花生粕纳豆菌发酵提取液,延缓雌性大鼠生殖系统衰老以及改善抗生素诱导型小鼠腹泻的作用。实验一:花生粕发酵物延缓雌鼠生殖系统衰老的作用研究建立雌性大鼠衰老模型,将72只雌性SD大鼠,按体重随机区组分为:空白对照组N(生理盐水)、模型组M(生理盐水)、低剂量组FL(发酵物25倍稀释液)、中剂量组FM(发酵物5倍稀释液)、高剂量组FH(发酵物原液)和阳性对照组Y(VE100 mg/kg),每组12只。除空白对照组腹腔注射生理盐水外,其余各组均腹腔注射D-半乳糖(400 mg/kg.d),灌胃剂量按照0.2 ml/10 g.d。实验进行8周后,处死动物,计算脏器指数,并对样本进行组织学观察和性激素等指标测定。结果显示,FM组和Y组的子宫指数与卵巢指数均高于M组(P0.05)。FM组、FH组血清雌二醇(E2)含量显著高于M组(P0.05)。FM组、FH组、Y组血清促卵泡生成素(FSH)含量显著低于M组(P0.05)。各处理组血清促黄体生成素(LH)含量均显著低于M组,且有统计学意义(P0.05)。结论:花生粕纳豆菌发酵物具有抗氧化作用,中、高剂量的花生粕发酵物能延缓雌性大鼠生殖系统衰老。实验二:花生粕发酵物改善小鼠腹泻的作用研究将40只昆明小鼠按照体重随机区组分为空白对照组A(生理盐水)、模型组B(氨苄西林)、自然恢复组C(氨苄西林+生理盐水)和HFY组D(氨苄西林+花生粕发酵液)。除空白对照组(A)外,各处理组均每天灌胃氨苄西林(剂量按2 mg/g bw·d,相当于成人剂量的5倍),直至各组出现腹泻,造模结束,处死模型组(B)小鼠并收集肠道内容物。空白对照组(A)和自然恢复组(C)灌胃生理盐水,HFY组(D)灌胃发酵原液,剂量0.2 ml/10 g.d,连续灌胃5天处死小鼠,收集肠道内容物。依托Illumina Hi Seq2500测序平台,对肠道内容物进行高通量测序,探究肠道内容物中菌群的分类及丰度。结果显示,在门的水平上,各实验组排在前三位的物种为拟杆菌门、厚壁菌门和变形菌门,其中拟杆菌门相对含量依次为B组(79.87%)、C组(65.90%)、A组(58.22%)和D组(45.10%)。厚壁菌门各组由高到低的顺序为D、A、C、B。变形菌门的相对含量由高到低依次为B组(17.73%)、C组(17.60%)、A组(11.63%)和D组(6.92%)。在属的水平上,B组除志贺菌属外,其他均呈现较低的水平。D组毛螺菌科NK4A136属和罗斯氏菌均高于其他各组;梭菌属D组高于B组;粪球菌属A、D两组均高于B组;乳杆菌属D组高于A、B两组;肠球菌属D组高于A组;多样性指数的组间差异显示,B组与各组都存在菌种丰度的差异(P0.05),D组的物种丰度高于其他三组(P0.05)。该结果表明花生粕发酵物对抗生素诱导腹泻小鼠的肠道菌群有一定的调节作用,可增加腹泻小鼠肠道有益菌的数量,抑制有害菌的生长,增加肠道菌群的丰度和物种均匀度。
[Abstract]:In this study, peanut meal was used as the main raw material for solid fermentation of peanut meal by Bacillus natto. The effects of fermentation extract of natto peanut meal on delaying senescence of reproductive system in female rats and improving antibiotic-induced diarrhea in mice were investigated. Experiment one: the effect of peanut meal ferment on delaying the senescence of reproductive system in female rats; 72 female SD rats were randomly divided into blank control group N (normal saline) according to body weight and 72 female rats were randomly divided into two groups: control group N (NS), control group N (NS), Model group M (saline), low dose group FL (fermentation material 25 times diluent), medium dose group FM (fermentation material 5 times dilution liquid), high dose group FH (fermentation material original liquor) and positive control group Y (VE100 mg/kg), each group 12 rats. Except for the blank control group, the other groups were intraperitoneally injected with D-galactose (400 mg/kg.d), and the gastric dose was 0.2 ml/10 g 路d. Eight weeks after the experiment, the animals were killed, the organ index was calculated, and the histological observation and sex hormone were performed on the samples. The results showed that the uterine index and ovarian index in FM and Y groups were significantly higher than those in M group (P0.05). FM group, FH group and M group, P 0.05). FM group and FH group, respectively). The level of serum follicle stimulating hormone (FSH) in group Y was significantly lower than that in group M (P0.05). The levels of serum luteinizing hormone (LH) in each treatment group were significantly lower than those in M group (P0.05). Conclusion: the ferment of peanut meal natto has antioxidation effect, and the high dose of peanut meal ferment can delay the senescence of reproductive system in female rats. Experiment 2: effects of peanut meal ferment on diarrhea in mice; 40 Kunming mice were divided into blank control group A (saline), model group B (ampicillin) according to the weight of mice randomly divided into two groups: control group A (normal saline), model group B (ampicillin), Natural recovery group C (ampicillin saline) and HFY group D (ampicillin peanut meal fermentation broth). With the exception of the blank control group (A), all the treatment groups were administered with ampicillin every day (2 mg/g bw 路d, equivalent to 5 times the adult dose) until diarrhea occurred in each group and the model was completed. The (B) mice in the model group were killed and the intestinal contents were collected. (A) in blank control group, (C) in natural recovery group, (D) in HFY group were fed intragastrically with 0. 2 ml/10 路d, the mice were killed for 5 days, and the contents of intestinal tract were collected. Based on Illumina Hi Seq2500 sequencing platform, high-throughput sequencing of intestinal contents was carried out to explore the taxonomy and abundance of flora in intestinal contents. The results showed that at the gate level, the first three species in each experimental group were Bacteroides, thick-walled bacteria and Proteus. The relative content of Bacteroides in Group B (79.87%), C group, 65.90%) was in order, and the relative content of Bacteroides in Group B (79.87%) (65.90%) was the highest. Group A (58.22%) and Group D (45.10%). The order from high to low in each group of thick-walled fungi was D, A, C, B. The relative content of Proteus was in the order of group B (17.73%), C) (17.60%), A, 11.63%) and group D (6.92%) from high to low. NK4A136 and Rossiella in Group D were higher than those in other groups, Clostridium group D was higher than Group B, Group A and Group D were higher than Group B, and Group D was higher than Group B. Lactobacillus D group was higher than A group, B group, Enterococcus group D group was higher than A group; The differences of diversity index showed that there were differences in species abundance between group B and group B (P0.05). The species abundance of), D group was higher than that of the other three groups (P0.05). The results showed that peanut meal ferment could regulate the intestinal flora of diarrhea mice induced by antibiotics, increase the number of intestinal beneficial bacteria, inhibit the growth of harmful bacteria, and increase the abundance of intestinal flora and species evenness.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R151
本文编号：2442039
[Abstract]:In this study, peanut meal was used as the main raw material for solid fermentation of peanut meal by Bacillus natto. The effects of fermentation extract of natto peanut meal on delaying senescence of reproductive system in female rats and improving antibiotic-induced diarrhea in mice were investigated. Experiment one: the effect of peanut meal ferment on delaying the senescence of reproductive system in female rats; 72 female SD rats were randomly divided into blank control group N (normal saline) according to body weight and 72 female rats were randomly divided into two groups: control group N (NS), control group N (NS), Model group M (saline), low dose group FL (fermentation material 25 times diluent), medium dose group FM (fermentation material 5 times dilution liquid), high dose group FH (fermentation material original liquor) and positive control group Y (VE100 mg/kg), each group 12 rats. Except for the blank control group, the other groups were intraperitoneally injected with D-galactose (400 mg/kg.d), and the gastric dose was 0.2 ml/10 g 路d. Eight weeks after the experiment, the animals were killed, the organ index was calculated, and the histological observation and sex hormone were performed on the samples. The results showed that the uterine index and ovarian index in FM and Y groups were significantly higher than those in M group (P0.05). FM group, FH group and M group, P 0.05). FM group and FH group, respectively). The level of serum follicle stimulating hormone (FSH) in group Y was significantly lower than that in group M (P0.05). The levels of serum luteinizing hormone (LH) in each treatment group were significantly lower than those in M group (P0.05). Conclusion: the ferment of peanut meal natto has antioxidation effect, and the high dose of peanut meal ferment can delay the senescence of reproductive system in female rats. Experiment 2: effects of peanut meal ferment on diarrhea in mice; 40 Kunming mice were divided into blank control group A (saline), model group B (ampicillin) according to the weight of mice randomly divided into two groups: control group A (normal saline), model group B (ampicillin), Natural recovery group C (ampicillin saline) and HFY group D (ampicillin peanut meal fermentation broth). With the exception of the blank control group (A), all the treatment groups were administered with ampicillin every day (2 mg/g bw 路d, equivalent to 5 times the adult dose) until diarrhea occurred in each group and the model was completed. The (B) mice in the model group were killed and the intestinal contents were collected. (A) in blank control group, (C) in natural recovery group, (D) in HFY group were fed intragastrically with 0. 2 ml/10 路d, the mice were killed for 5 days, and the contents of intestinal tract were collected. Based on Illumina Hi Seq2500 sequencing platform, high-throughput sequencing of intestinal contents was carried out to explore the taxonomy and abundance of flora in intestinal contents. The results showed that at the gate level, the first three species in each experimental group were Bacteroides, thick-walled bacteria and Proteus. The relative content of Bacteroides in Group B (79.87%), C group, 65.90%) was in order, and the relative content of Bacteroides in Group B (79.87%) (65.90%) was the highest. Group A (58.22%) and Group D (45.10%). The order from high to low in each group of thick-walled fungi was D, A, C, B. The relative content of Proteus was in the order of group B (17.73%), C) (17.60%), A, 11.63%) and group D (6.92%) from high to low. NK4A136 and Rossiella in Group D were higher than those in other groups, Clostridium group D was higher than Group B, Group A and Group D were higher than Group B, and Group D was higher than Group B. Lactobacillus D group was higher than A group, B group, Enterococcus group D group was higher than A group; The differences of diversity index showed that there were differences in species abundance between group B and group B (P0.05). The species abundance of), D group was higher than that of the other three groups (P0.05). The results showed that peanut meal ferment could regulate the intestinal flora of diarrhea mice induced by antibiotics, increase the number of intestinal beneficial bacteria, inhibit the growth of harmful bacteria, and increase the abundance of intestinal flora and species evenness.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R151
【参考文献】
相关期刊论文 前9条
1 刘瑞芳;赵华;李建华;程青虹;;益生菌酸奶对机体血脂水平影响的Meta分析[J];中国全科医学;2016年32期
2 周晓丹;刘爱萍;张明;郭慧媛;任发政;;副干酪乳杆菌LC-01对便秘小鼠的通便作用[J];乳业科学与技术;2012年05期
3 张常娥;魏伟;刘英华;谢静;;D-半乳糖亚急性衰老大鼠模型的建立及评价[J];中国老年学杂志;2012年04期
4 蒋万志;张洪泉;;枸杞多肽对D-半乳糖诱导小鼠的抗衰老作用及其可能机制[J];国际药学研究杂志;2010年01期
5 郭世奎;包维民;龚昆梅;胡大春;邵剑春;陈弟;王昆华;;实时荧光定量PCR法研究结直肠癌患者肠道拟杆菌属、梭杆菌属和梭菌属量的变化[J];中国微生态学杂志;2010年01期
6 杜瑞亭;托娅;张和平;;干酪乳杆菌Zhang对小鼠肠道免疫功能的影响[J];营养学报;2009年04期
7 徐营,李霞,杨利国;双歧杆菌的生物学特性及对人体的生理功能[J];微生物学通报;2001年06期
8 李文彬，，韦丰，范明，张京立，张炳烈，马向晨，杨卫平，魏文;D－半乳糖在小鼠上诱导的拟脑老化效应[J];中国药理学与毒理学杂志;1995年02期
9 龚国清,徐黻本;小鼠衰老模型研究[J];中国药科大学学报;1991年02期
相关博士学位论文 前1条
1 余欣慧;益肾续经汤延缓绝经过渡期大鼠卵巢功能衰老的实验研究[D];湖北中医药大学;2010年
相关硕士学位论文 前1条
1 张文娣;抗生素相关性腹泻模型的构建[D];南方医科大学;2015年
本文编号：2442039
本文链接：https://www.wllwen.com/yixuelunwen/yufangyixuelunwen/2442039.html
