低碘环境对子代大鼠血清及睾丸抗氧化能力的影响

发布时间：2019-03-22 19:12

【摘要】：目的研究不同低碘含量饲料对Wistar子代雄鼠血清及睾丸抗氧化能力的影响。方法对SPF级Wistar雌鼠长期喂饲碘含量分别为20μg/kg(A组)、50μg/kg(B组)、300μg/kg(C组)饲料,检测"三代两窝"传代后子二代喂养3个月、6个月、9个月时雄鼠血清、睾丸组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)的含量。结果从亲代开始长期进食低碘饲料明显影响子二代雄鼠血清及睾丸抗氧化能力,且饲料碘含量越低影响越明显。血清SOD、睾丸组织GSH-Px受低碘影响最显著(P0.05)。进一步分析造成睾丸抗氧化能力降低的因素,结果表明与低碘影响睾丸组织细胞线粒体SOD活性有关(P0.05)。结论随低碘影响周期越长,机体碘营养水平越差,对子代抗氧化能力影响越明显,说明碘缺乏干预应在早期进行,也提示适时进行碘缺乏高危对象抗氧化能力检测可作为判断碘缺乏损伤的指标之一。
[Abstract]:Objective to study the effects of low iodine diet on the antioxidation ability of serum and testis of male Wistar offspring. Methods the female rats of SPF grade Wistar were fed with iodine content of 20 渭 g / kg (A group, 50 渭 g / kg (B group and 300 渭 g / kg (C group for a long time. The serum of male rats were fed for 3 months, 6 months and 9 months after the passage of "three generations, two nests", and the second generation was fed for 3 months, 6 months and 9 months, respectively. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content in testis. Results long-term intake of iodine-deficient diet from parents significantly affected the antioxidation ability of serum and testes of the second generation male rats, and the lower the iodine content in feed was, the more obvious the effect was. Serum SOD, testicular tissue GSH-Px was most affected by iodine deficiency (P0.05). The results showed that iodine deficiency affected mitochondrial SOD activity of testicular cells (P0.05). Conclusion the longer the cycle of iodine deficiency is, the worse the iodine nutrition level is, and the more obvious the effect on the antioxidation ability of offspring is, which indicates that the intervention of iodine deficiency should be carried out in the early stage. It is also suggested that the detection of antioxidant capacity in high-risk patients with iodine deficiency can be used as one of the indexes to judge iodine deficiency damage.
【作者单位】：兰州大学公共卫生学院营养与食品卫生学研究所;新疆维吾尔自治区疾病预防控制中心;兰州市疾病预防控制中心;
【基金】：国家自然科学基金项目(81160334) 兰州大学大学生创新创业项目(201310730136)~~
【分类号】：R153.1

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