饮用水中2,6-二氯-1,4-苯醌的体内急性毒性和遗传毒性研究

发布时间：2019-03-28 12:11

【摘要】：目的研究饮用水中2,6-二氯-1,4-苯醌(2,6-DCBQ)对小鼠的急性毒性和遗传毒性作用。方法选择健康6~8周龄SPF级昆明小鼠进行试验。将50只小鼠随机分为5组,分别为阴性对照(玉米油)组和215、464、1 000、2 150 mg/kg 2,6-DCBQ染毒组,每组10只,雌雄各半。采用一次性经口灌胃方式进行染毒,染毒容量为20 ml/kg。染毒后观察14 d,记录动物死亡数,计算其半数致死剂量(median lethal dose,LD50)。将50只小鼠随机分为5组,分别为溶剂对照(玉米油)组和62.5、125和250 mg/kg 2,6-DCBQ染毒组及阳性对照组(40 mg/kg环磷酰胺),每组10只,雌雄各半。采用灌胃方式进行染毒,染毒容量为20 ml/kg,两次灌胃间隔24 h;第二次灌胃后6 h,进行骨髓细胞微核试验。将50只雄性小鼠随机分为5组,分别为溶剂对照(玉米油)组和62.5、125和250 mg/kg 2,6-DCBQ染毒组及阳性对照组(40 mg/kg环磷酰胺),每组10只。采用经口灌胃方式进行染毒,染毒容量为20 ml/kg,连续灌胃5 d。染毒后第35天,进行精子畸形试验。结果 2,6-DCBQ对雄性、雌性小鼠经口急性毒性的LD50分别为501 mg/kg(95%CI:344~730 mg/kg)和584 mg/kg(95%CI:430~794mg/kg)。不同剂量2,6-DCBQ染毒组小鼠的骨髓细胞微核率及精子畸形率与溶剂对照组比较,差异均无统计学意义(P0.05)。各组PCE/NCE值均在正常范围内。结论 2,6-DCBQ对小鼠经口急性毒性属于低毒级,且未发现其具有遗传毒性。
[Abstract]:Aim to study the acute and genotoxic effects of 2,6-dichloro-1,4-benzoquinone (2,6-DCBQ) in drinking water on mice. Methods healthy 6-8-week-old SPF Kunming mice were tested. 50 mice were randomly divided into 5 groups: negative control (corn oil) group, 215,464, 1 000, 2 150 mg/kg 2, 6 d DCBQ group (n = 10, male and female). One-time oral administration was carried out with a capacity of 20 ml/kg.. The mortality of animals was recorded and the lethal dose (median lethal dose,LD50) was calculated for 14 days after exposure. 50 mice were randomly divided into 5 groups: solvent control (corn oil) group, 62.5125 and 250 mg/kg 2 groups, 6 DCBQ exposure group and positive control group (40 mg/kg cyclophosphamide), with 10 mice in each group, half male and half female. The dosage was 24 hours after 20 ml/kg, and the micronucleus test of bone marrow cells was carried out 6 hours after the second gavage. Fifty male mice were randomly divided into 5 groups: solvent control (corn oil) group, 62.5125 and 250 mg/kg 2 groups, 6 DCBQ exposure group and positive control group (40 mg/kg cyclophosphamide), with 10 mice in each group. Oral administration was carried out by oral administration with a capacity of 20 ml/kg, for 5 d. Sperm deformity test was performed on the 35th day after exposure. Results 2. The LD50 of acute oral toxicity to male and female mice was 501 mg/kg (95%CI:344~730 mg/kg) and 584 mg/kg (95%CI:430~794mg/kg), respectively. There was no significant difference in micronucleus rate and sperm deformity rate of bone marrow cells between 2 and 6 DCBQ groups compared with solvent control group (P0.05). The values of PCE/ NCE in each group were within the normal range. Conclusion 2. The acute toxicity of 6 DCBQ to mice was low, and no genotoxicity was found.
【作者单位】：广西医科大学公共卫生学院职业卫生与环境卫生系;广西壮族自治区疾病预防控制中心卫生毒理与功能检验所;
【基金】：国家自然科学基金(81360421;81560524) 中国博士后科学基金(2013M540686;2014T70839) 广西自然科学基金(2012GXNSFBA053109) 广西高等学校优秀中青年骨干教师培养工程
【分类号】：R123

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