PM2.5染尘大鼠海马中铅铝锰含量及神经递质的变化

发布时间：2019-04-10 08:53

【摘要】：为探讨吸入大气PM2.5的大鼠海马组织和全血中铅、铝、锰含量变化及血清和海马中神经递质一氧化氮(NO)和一氧化氮合酶(NOS)的变化,将SPF级雄性大鼠32只随机分为对照组、PM2.5低剂量组(5 mg/kg)、PM2.5中剂量组(10 mg/kg)、PM2.5高剂量组(20 mg/kg),每组8只,经气管注入PM2.5混悬液,每周1次,连续12周。测定大脑海马组织和全血中铅、铝、锰含量及血清、海马中NO含量和NOS活力。结果显示,随着染毒剂量的增加,大鼠海马中铅含量逐渐升高,中、高剂量组海马中锰含量和高剂量组海马中铝含量高于对照组,高剂量组血清NO含量和各染毒组海马组织中NO含量低于对照组,高剂量组海马组织中NOS活力低于对照组,差异均有统计学意义(P0.05)。提示PM2.5可使大鼠海马中铅、铝、锰含量升高,海马NO含量和NOS活力降低。
[Abstract]:To investigate the changes of lead, aluminum and manganese contents in hippocampus and whole blood and the changes of nitric oxide (NO) and nitric oxide synthase (NOS) in serum and hippocampus of rats inhaled with atmospheric PM2.5. Thirty-two male SPF rats were randomly divided into control group, PM2.5 low dose group (5 mg/kg), PM2.5 medium dose group (10 mg/kg), PM2.5 high dose group (20 mg/kg), 8 rats in each group. PM2.5 suspension was injected via trachea. Once a week for 12 consecutive weeks. The contents of lead, aluminum and manganese in hippocampus and whole blood, the content of NO and the activity of NOS in serum and hippocampus were measured. The results showed that the content of lead in hippocampus of rats increased gradually with the increase of exposure dose. The content of manganese in hippocampus of middle and high dose group and aluminum content of hippocampus of high dose group were higher than those of control group. The content of serum NO and the content of NO in hippocampus of the high-dose group were lower than those of the control group, and the activity of NOS in the high-dose group was lower than that of the control group, the difference was statistically significant (P0.05). It was suggested that PM2.5 could increase the contents of lead, aluminum and manganese in the hippocampus of rats, and decrease the content of NO and the activity of NOS in the hippocampus of rats.
【作者单位】：唐山市环境监测中心站大气监测室;河北联合大学公共卫生学院;
【分类号】：R114

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