2,4-滴丁酯染毒对大鼠学习记忆行为及氨基酸类神经递质含量的影响

发布时间：2019-04-13 11:44

【摘要】：[目的]探讨2,4-滴丁酯90 d染毒对雄性大鼠学习记忆行为及大脑皮质、海马组织中氨基酸类神经递质含量的影响。[方法]将40只健康30 d龄SPF级雄性Wistar大鼠随机分为4组,分别为对照组(玉米油∶蒸馏水体积比为1∶1)和2,4-滴丁酯低(1/100 LD50,6 mg/kg)、2,4-滴丁酯中(3/100 LD50,18 mg/kg)、2,4-滴丁酯高(6/100 LD50,36 mg/kg)剂量组,每组10只。经口灌胃染毒,每周连续染毒5 d,实验周期为90 d。染毒第4、8、13周进行Morris水迷宫测试大鼠的学习记忆能力。染毒结束后,高效液相色谱法检测大鼠大脑皮质、海马中谷氨酸、天门冬氨酸、γ-氨基丁酸、甘氨酸的含量。[结果]Morris水迷宫测试第13周时,随染毒剂量升高,大鼠平均逃避潜伏期逐渐延长(P0.05),呈剂量-效应关系(r=0.961 1,P0.01)。随着2,4-滴丁酯染毒剂量的升高,大鼠大脑皮质中谷氨酸水平呈升高趋势(P0.05)、γ-氨基丁酸水平呈下降趋势(P0.05),均呈剂量-效应关系(r=0.942 5,r=-0.887 2,均P0.01);天门冬氨酸含量在高剂量组升高显著(P0.05)。海马中谷氨酸含量、天门冬氨酸含量均呈升高趋势(P0.05)、γ-氨基丁酸含量呈下降趋势(P0.05),均呈剂量-效应关系(r=0.970 1,r=0.886 6,r=-0.916 2,均P0.01)。[结论]2,4-滴丁酯亚慢性染毒影响大鼠学习记忆能力及大鼠脑组织氨基酸类神经递质水平。
[Abstract]:[aim] to investigate the effects of 2,4-butyl dibutyl on learning and memory behavior and the content of amino acid neurotransmitters in cerebral cortex and hippocampus of male rats. [methods] 40 healthy 30-day-old male Wistar rats were randomly divided into 4 groups: control group (corn oil: distilled water volume ratio was 1:1) and 2,4-dibutyl low (1 渭 100 LD50,6 mg/kg). 2, 4-butyl dibutyl group (3 渭 100 LD50,18 mg/kg), 2,4-butyl dibutyl high dose group (6 渭 100 LD50,36 mg/kg), 10 rats in each group (n = 10, n = 10). Oral administration was given to the stomach for 5 days a week, and the experiment period was 90 d. The learning and memory abilities of rats were tested by Morris water maze at 4,8 and 13 weeks after exposure. After exposure, the contents of glutamic acid, aspartate, 纬-aminobutyric acid and glycine in cerebral cortex and hippocampus of rats were determined by high performance liquid chromatography (HPLC). [results] at the 13th week of Morris water maze test, the mean escape latency of rats gradually prolonged with the increase of dose (P0.05), which showed a dose-effect relationship (r = 0.961 1, P0.01). With the increasing dose of 2,4-dibutyl, the level of glutamic acid in the cerebral cortex increased (P0.05), and the level of 纬-aminobutyric acid decreased (P0.05), both in a dose-effect relationship (r = 0.942 5, P < 0.01, P < 0.01), but the level of 纬-aminobutyric acid (r = 0.942 5, P < 0.05). (r = 0.887 2, P0.01); The content of aspartic acid increased significantly in the high dose group (P0.05). The content of glutamic acid and aspartic acid in hippocampus increased (P0.05), and the content of 纬-aminobutyric acid decreased (P0.05), and all showed a dose-effect relationship (r = 0.9701, r = 0.886 6, r = 0.916 2, P < 0.01), while the content of 纬-aminobutyric acid decreased (P < 0.05). (P0.01). [conclusion] 2, 4-dibutyl subchronic exposure affects the learning and memory ability of rats and the levels of amino acid neurotransmitters in brain tissue of rats.
【作者单位】：佳木斯大学公共卫生学院;
【基金】：黑龙江省教育厅科学技术研究项目(编号:12541789)
【分类号】：R114

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