锰对SH-SY5Y细胞线粒体超微结构及功能改变的研究

发布时间：2019-04-26 12:42

【摘要】：目的:探讨锰染毒对SH-SY5Y细胞线粒体超微结构的影响及线粒体功能的改变。方法:透射电镜观察对照组和MnCl_2处理组(250μmol/L,6 h)SH-SY5Y细胞线粒体形态的变化,流式细胞术检测细胞膜电位变化,以及荧光酶标仪检测细胞内活性氧的含量。结果:与对照组相比,MnCl_2处理组线粒体形态发生明显的肿胀、空泡化、基质浓缩明显,线粒体嵴数减少或消失;细胞线粒体膜电位在250μmol/L作用6 h后明显降低(P0.05);同时在相同处理条件下,ROS产生水平较对照组有明显升高(P0.05)。结论:锰可以损伤线粒体的超微结构并影响其功能。
[Abstract]:Aim: to investigate the effect of manganese exposure on mitochondrial ultrastructure and mitochondrial function in SH-SY5Y cells. Methods: the mitochondrial morphology of SH-SY5Y cells was observed by transmission electron microscopy (TEM), the changes of cell membrane potential by flow cytometry and the content of reactive oxygen species (Ros) in SH-SY5Y cells treated with MnCl_2 (250 渭 mol / L, 6 h). Results: compared with the control group, the mitochondria of MnCl_2 treated group had obvious swelling, vacuolization and matrix concentration, and the number of mitochondria cristae decreased or disappeared. The mitochondrial membrane potential was significantly decreased at 250 渭 mol / L for 6 h (P0.05), and the level of ROS production was significantly higher than that of the control group under the same treatment (P0.05). Conclusion: manganese can damage the ultrastructure of mitochondria and affect its function.
【作者单位】：首都医科大学公共卫生学院环境毒理学北京市重点实验室;
【基金】：国家自然科学基金项目(编号:81673137) 北京市自然科学基金项目(编号:7172024) 北京市教育委员会高等学校高层次人才引进与培养及创新团队建设计划项目(编号:CIT&TCD201504094)
【分类号】：R114

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