偏二甲肼对雄性小鼠生殖损伤的研究
发布时间：2019-05-05 06:34
【摘要】：目的 研究偏二甲肼对雄性小鼠是否具有生殖损伤，探讨偏二甲肼作用下小鼠精子质量和活力以及遗传改变并分析其可能的原因。 方法 实验选择健康成熟雄性昆明小鼠20只，随机分为4组，每组5只,即：正常对照组（NS组），偏二甲肼（UDMH）低剂量染毒组20mg/kg、中剂量染毒组40mg/kg、高剂量染毒组80mg/kg，称重，灌胃染毒，2次/周，连续8周。染毒结束次日，颈椎脱臼处死小鼠，分别制备检测样本，进行下列实验：精子质量检测以及精子活力检测；单细胞凝胶电泳实验。 结果 精子活力检测结果显示，和对照组相比，偏二甲肼作用下精子活力与对照组相比改变不大，未见精子明显活力减弱现象（P0.05）；单细胞凝胶电泳实验中，精子细胞被染成橘红色，，大部分呈正常形态且细胞核无拖尾现象，不同剂量组之间，拖尾细胞数差异不明显（P0.05）。 结论 未见偏二甲肼对小鼠精子数量减少和活力减低有明显相关。单细胞凝胶电泳(SCGE)实验未见偏二甲基肼对小鼠精子细胞DNA损伤有明显相关。
[Abstract]:Aim to study the reproductive damage of unsymmetrical dimethylhydrazine (UDMH) in male mice, to explore the sperm quality, motility and genetic changes under the action of UDMH and to analyze its possible causes. Methods Twenty healthy adult male Kunming mice were randomly divided into 4 groups: normal control group (NS group), unsymmetrical dimethylhydrazine (UDMH) low dose group (20mg / kg), middle dose group (40mg / kg), unsymmetrical dimethylhydrazine (UDMH) group (20 mg / kg) and middle dose group (40 mg / kg / kg). The high dose group was given 80 mg / kg, weighing, twice a week for 8 weeks. The mice were killed the next day after exposure to dislocated cervical vertebra. The test samples were prepared and the following experiments were carried out: sperm quality test and sperm motility test; single cell gel electrophoresis (SCGE) test. Results the results of sperm motility test showed that compared with the control group, the sperm motility under the action of unsymmetrical dimethylhydrazine had no significant change compared with the control group, and there was no obvious decrease in sperm motility (P0.05). In single cell gel electrophoresis (SCGE), sperm cells were stained orange-red, most of them were in normal shape and the nucleus had no tail-dragging phenomenon. There was no significant difference in the number of tail-tailed cells between different dose groups (P0.05). Conclusion there is no significant correlation between unsymmetrical dimethylhydrazine (UDMH) and decreased sperm motility in mice. Single cell gel electrophoresis (SCGE) showed no significant correlation between unsymmetrical dimethylhydrazine (UDMH) and DNA damage in mouse spermatozoa.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R114
[Abstract]:Aim to study the reproductive damage of unsymmetrical dimethylhydrazine (UDMH) in male mice, to explore the sperm quality, motility and genetic changes under the action of UDMH and to analyze its possible causes. Methods Twenty healthy adult male Kunming mice were randomly divided into 4 groups: normal control group (NS group), unsymmetrical dimethylhydrazine (UDMH) low dose group (20mg / kg), middle dose group (40mg / kg), unsymmetrical dimethylhydrazine (UDMH) group (20 mg / kg) and middle dose group (40 mg / kg / kg). The high dose group was given 80 mg / kg, weighing, twice a week for 8 weeks. The mice were killed the next day after exposure to dislocated cervical vertebra. The test samples were prepared and the following experiments were carried out: sperm quality test and sperm motility test; single cell gel electrophoresis (SCGE) test. Results the results of sperm motility test showed that compared with the control group, the sperm motility under the action of unsymmetrical dimethylhydrazine had no significant change compared with the control group, and there was no obvious decrease in sperm motility (P0.05). In single cell gel electrophoresis (SCGE), sperm cells were stained orange-red, most of them were in normal shape and the nucleus had no tail-dragging phenomenon. There was no significant difference in the number of tail-tailed cells between different dose groups (P0.05). Conclusion there is no significant correlation between unsymmetrical dimethylhydrazine (UDMH) and decreased sperm motility in mice. Single cell gel electrophoresis (SCGE) showed no significant correlation between unsymmetrical dimethylhydrazine (UDMH) and DNA damage in mouse spermatozoa.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R114
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1 金龙金,楼哲丰,董杰影,陈锡文;汞、镉对小鼠离体骨髓细胞和睾丸生殖细胞的DNA损伤作用[J];癌变.畸变.突变;2004年02期
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