表面解吸常压化学电离质谱法在肉品质与龋齿分析中的应用研究
发布时间：2019-05-25 01:35
【摘要】：绿色、原位、快速、无损检测技术的开发是分析行业新的发展方向。近年来常压快速质谱技术以突破传统质谱分析需冗长预处理和分离的限制,及实现样品的原位、实时、在线、非破坏和低耗损的质谱分析,得以迅速发展。表面解吸常压化学电离质谱(DAPCI-MS)技术是新兴的常压快速质谱技术之一,可在常温、常压、无解吸溶剂的条件下对未经样品预处理的各种复杂样品表面痕量物质进行解吸电离,对样品无污染、无破坏,是对实际样品原位质谱检测较为理想的电离方法之一。本文主要研究DAPCI-MS在原料肉品质及龋齿表面微区分析中的应用。本文的主要研究内容包括:1.DAPCI-MS技术在猪肉新鲜度评价中的应用。在无需样品预处理的条件下,采用DAPCI-MS方法直接获取猪肉新鲜度随时间变化的质谱指纹谱图,采用串联质谱技术对关键目标化合物进行了初步的分子式鉴定,并结合PCA和CA方法,分别对不同新鲜度的原料肉进行区分及方法的可重现性分析。结果表明:冷藏保存1~8天猪肉的质谱指纹谱图明显分为三类,第一类为1~3天的质谱图,是新鲜度变化的初期,第二类为4~6天的质谱图,是新鲜度向腐败变化的过渡时期,第三类为7~8天的质谱图,是品质完全发生变化的时期。该方法简单、快速,是分析复杂基体样品的一种有效实用的分析手段。2.DAPCI-MS技术在原料肉掺假分析中的应用。选择4种原料肉和3种非同源掺假肉进行DAPCI-MS分析,结合PCA方法,对真假肉进行区分;采用未知真伪的肉源样品进行验证,结果匹配。该方法对基体复杂的肉类样品具有较好的分析能力,单个样品的分析时间不超过30 s,原料肉和掺假肉的质谱指纹谱图之间存在差异,结合主成分分析(PCA)法能有效地将原料肉和掺假肉区分开。3.龋齿表面微区的DAPCI-MS分析。采用纳升取样DAPCI-MS直接对龋齿不同部位取样并进行快速质谱分析,结合PCA方法,对龋齿病灶位置与邻近正常组织进行区分,以及牙病与健康牙齿的区分;结合串联质谱可快速检定龋齿中的乳酸、丙酮酸、苯乙酸和丙酸等关键成分。该方法及装置可方便地对牙齿进行直接微区分析,为鉴别牙齿疾病、观测治疗效果提供了一种快速、简单的方法,为生物体中微细部位的快速取样及直接质谱分析提供了一种可能的解决方案。
[Abstract]:The development of green, in situ, fast and nondestructive testing technology is a new development direction of analysis industry. In recent years, atmospheric pressure rapid mass spectrometry has been developed rapidly to break through the limitation of long pretreatment and separation of traditional mass spectrometry, and to realize in situ, real-time, on-line, non-destructive and low loss mass spectrometry of samples. Surface desorption atmospheric pressure chemical ionization mass spectrometry (DAPCI-MS) is one of the new atmospheric pressure rapid mass spectrometry techniques, which can be used at room temperature and atmospheric pressure. Desorption and ionization of trace substances on the surface of various complex samples without desorption solvent is one of the ideal ionization methods for in situ mass spectrometry of actual samples. In this paper, the application of DAPCI-MS in raw meat quality and dental caries surface microanalysis was studied. The main research contents of this paper include: the application of 1.DAPCI-MS technology in pork freshness evaluation. Under the condition of no sample pretreatment, the mass spectrometry fingerprint of pork freshness changed with time was obtained by DAPCI-MS method, and the key target compounds were identified by tandem mass spectrometry. Combined with PCA and CA methods, the raw meat with different freshness was distinguished and the reproducibility of the method was analyzed. The results showed that the mass spectrometry fingerprints of pork stored in cold storage for 1 脳 8 days were obviously divided into three categories. The first type was 1 脳 3 days mass spectrometry, which was the initial stage of freshness change, and the second type was 4 脳 6 days mass spectrometry, which was the transitional period from freshness to corruption. The third type is 7 脳 8 days mass spectrometry, which is a period of complete quality change. This method is simple and rapid, and it is an effective and practical method for the analysis of complex matrix samples. Application of 2.DAPCI-MS technique in adulteration Analysis of Raw Meat. Four kinds of raw meat and three kinds of non-homologous adulterated meat were selected for DAPCI-MS analysis, combined with PCA method, the true and false meat was distinguished, and the unknown true and false meat source samples were used to verify the results, and the results were matched. This method has good analytical ability for meat samples with complex matrix. The analysis time of a single sample is not more than 30 s. There is a difference in mass spectrometry fingerprint between raw meat and adulterated meat. Combined with principal component analysis (PCA), (PCA) can effectively distinguish raw meat from adulterated meat. DAPCI-MS analysis of microarea on the surface of caries. DAPCI-MS was used to directly sample different parts of dental caries and rapid mass spectrometry analysis was carried out. Combined with PCA method, the location of caries lesions was distinguished from adjacent normal tissues, as well as the distinction between dental diseases and healthy teeth. Lactic acid, pyruvate, phenylacetic acid and propionic acid in dental caries can be quickly identified by tandem mass spectrometry. The method and device can easily analyze the teeth directly, which provides a rapid and simple method for distinguishing dental diseases and observing the therapeutic effect. It provides a possible solution for rapid sampling and direct mass spectrometry analysis of fine parts in organisms.
【学位授予单位】：东华理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R155.5;TS207.3;O657.63
本文编号：2485375
[Abstract]:The development of green, in situ, fast and nondestructive testing technology is a new development direction of analysis industry. In recent years, atmospheric pressure rapid mass spectrometry has been developed rapidly to break through the limitation of long pretreatment and separation of traditional mass spectrometry, and to realize in situ, real-time, on-line, non-destructive and low loss mass spectrometry of samples. Surface desorption atmospheric pressure chemical ionization mass spectrometry (DAPCI-MS) is one of the new atmospheric pressure rapid mass spectrometry techniques, which can be used at room temperature and atmospheric pressure. Desorption and ionization of trace substances on the surface of various complex samples without desorption solvent is one of the ideal ionization methods for in situ mass spectrometry of actual samples. In this paper, the application of DAPCI-MS in raw meat quality and dental caries surface microanalysis was studied. The main research contents of this paper include: the application of 1.DAPCI-MS technology in pork freshness evaluation. Under the condition of no sample pretreatment, the mass spectrometry fingerprint of pork freshness changed with time was obtained by DAPCI-MS method, and the key target compounds were identified by tandem mass spectrometry. Combined with PCA and CA methods, the raw meat with different freshness was distinguished and the reproducibility of the method was analyzed. The results showed that the mass spectrometry fingerprints of pork stored in cold storage for 1 脳 8 days were obviously divided into three categories. The first type was 1 脳 3 days mass spectrometry, which was the initial stage of freshness change, and the second type was 4 脳 6 days mass spectrometry, which was the transitional period from freshness to corruption. The third type is 7 脳 8 days mass spectrometry, which is a period of complete quality change. This method is simple and rapid, and it is an effective and practical method for the analysis of complex matrix samples. Application of 2.DAPCI-MS technique in adulteration Analysis of Raw Meat. Four kinds of raw meat and three kinds of non-homologous adulterated meat were selected for DAPCI-MS analysis, combined with PCA method, the true and false meat was distinguished, and the unknown true and false meat source samples were used to verify the results, and the results were matched. This method has good analytical ability for meat samples with complex matrix. The analysis time of a single sample is not more than 30 s. There is a difference in mass spectrometry fingerprint between raw meat and adulterated meat. Combined with principal component analysis (PCA), (PCA) can effectively distinguish raw meat from adulterated meat. DAPCI-MS analysis of microarea on the surface of caries. DAPCI-MS was used to directly sample different parts of dental caries and rapid mass spectrometry analysis was carried out. Combined with PCA method, the location of caries lesions was distinguished from adjacent normal tissues, as well as the distinction between dental diseases and healthy teeth. Lactic acid, pyruvate, phenylacetic acid and propionic acid in dental caries can be quickly identified by tandem mass spectrometry. The method and device can easily analyze the teeth directly, which provides a rapid and simple method for distinguishing dental diseases and observing the therapeutic effect. It provides a possible solution for rapid sampling and direct mass spectrometry analysis of fine parts in organisms.
【学位授予单位】：东华理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R155.5;TS207.3;O657.63
【共引文献】
相关期刊论文 前2条
1 李秀杰;滕国玲;余红;;基于聚类分析的消费者绿色羊肉购买行为研究[J];黑龙江畜牧兽医;2014年11期
2 陆昌华;胡肄农;甘泉;孙友珍;谭业平;郁达威;刘亮;田景福;储新民;;动物食品质量安全风险因素分析及其防控对策[J];江苏农业学报;2015年03期
相关博士学位论文 前3条
1 孙洪波;猪肉供应链上的机会主义根源及治理对策研究[D];中国农业科学院;2012年
2 费亚利;政府强制性猪肉质量安全可追溯体系研究[D];四川农业大学;2012年
3 刘万兆;基于封闭供应链的猪肉质量安全控制研究[D];沈阳农业大学;2013年
相关硕士学位论文 前1条
1 沈斌;大部制背景下我国猪肉类产品安全监管体系之完善研究[D];南昌大学;2015年
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