余氯对河流水体微生物灭活效应的评价

发布时间：2019-06-02 22:08

【摘要】：【目的】研究不同余氯浓度和暴露时间对细菌的去除效果,分析不同余氯条件对细胞ATP的影响。【方法】以河水中微生物群落为试验对象,利用流式细胞术(Flow cytometry,FCM)评估不同余氯浓度和暴露时间的灭活效果,检测不同余氯浓度时细胞内(外)ATP的变化情况。【结果】不同余氯浓度和暴露时间对细菌的去除效果产生不同的结果。在余氯浓度2 mg/L情况下,延长氯暴露时间可以增加细菌的去除效果,在余氯浓度≥2 mg/L条件下,较短氯暴露时间就可以灭活90%细菌。高核苷酸细菌(HNA)和低核苷酸细菌(LNA)表现出不同氯耐受能力,且HNA细菌相比LNA细菌较容易受到氯的损伤。细胞内ATP随余氯浓度增加而减少,在高浓度余氯条件下(≥2 mg/L)细胞外ATP才会增加。【结论】微生物活性随着余氯作用的增加而降低,FCM法和ATP检测法可以用于评估加氯消毒对微生物稳定性的影响。
[Abstract]:[Objective] To study the effect of different residual chlorine concentration and exposure time on the bacterial removal, and to analyze the effect of different residual chlorine on the cell ATP. [Methods] The effect of different residual chlorine concentration and time of exposure was assessed by flow cytometry (FCM), and the change of intracellular (external) ATP was detected by flow cytometry (FCM). [Results] The effects of different residual chlorine concentration and exposure time on the bacteria removal were different. Under the condition of the residual chlorine concentration of 2 mg/ L, the removal effect of bacteria could be increased. Under the condition of the residual chlorine concentration of 2 mg/ L,90% of the bacteria could be inactivated by the shorter chlorine exposure time. The high-nucleonic acid bacteria (HNA) and the low-nuclear-acid bacteria (LNA) exhibited different chlorine tolerance, and the HNA bacteria were more susceptible to chlorine damage than the LNA bacteria. The intracellular ATP decreased with the increase of the residual chlorine concentration, and the ATP of the cells (2 mg/ L) increased with the increase of the residual chlorine concentration. [Conclusion] The microbial activity decreases with the increase of the residual chlorine, and the FCM method and the ATP detection method can be used to evaluate the effect of chlorination disinfection on the microbial stability.
【作者单位】：南开大学环境科学与工程学院环境污染过程与基准教育部重点实验室天津市城市生态环境修复与污染防治重点实验室;
【基金】：国家自然科学基金项目(No.31670498)~~
【分类号】：R123.6

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