芸豆芽菜多酚对氧化损伤小鼠体内抗氧化及肝脏损伤修复作用

发布时间：2019-06-04 22:14

【摘要】：本实验探究了芸豆芽菜多酚对D-半乳糖造模的氧化损伤小鼠体内抗氧化及肝脏损伤修复作用。腹腔注射D-半乳糖溶液构建氧化损伤小鼠模型,经连续灌胃不同剂量(20、40、60 mg/(kg·d))芸豆芽菜多酚28 d后测定小鼠体质量、肝脏脏器系数、血清超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate transaminase,AST)活力及丙二醛(malondialdehyde,MDA)含量并取肝脏制作苏木精-伊红染色切片,观察小鼠肝脏损伤及修复状况。与模型对照组小鼠比较,各灌胃给药组小鼠体质量及肝脏脏器系数无显著性差异(P0.05),芸豆芽菜多酚中、高剂量组小鼠血清SOD、GSH-Px活力显著上升(P0.05),MDA含量显著下降(P0.05),肝脏功能指示指标ALT与AST活力显著下降(P0.05),且综合肝组织病理学染色切片结果观察发现芸豆芽菜多酚可使肿胀的肝细胞恢复正常形态,并可减少胞间出血点及炎性细胞浸润现象,且作用效果与剂量呈正相关。
[Abstract]:The effects of polyphenols from kidney bean sprouts on oxidative damage induced by D-galactose in mice were studied. The mice model of oxidative injury was established by intraabdominal injection of D-galactose solution. The body mass and liver organ coefficient of the mice were measured after 20, 40, 60 mg/ (kg 路d) kidney bean sprouts polyphenols for 28 days. Serum Superoxide Dismutase (superoxide dismutase,SOD), Glutathion Peroxidase (glutathione peroxidase,GSH-Px), Glutamic pyruvic transaminase (alanine aminotransferase,ALT), glutamic oxaloacetic transaminase (aspartate transaminase,AST) and malondialdehyde (malondialdehyde,) The content of MDA and the section of hematoxylin and eosin staining were taken from the liver to observe the liver injury and repair in mice. Compared with the model control group, there was no significant difference in body mass and liver organ coefficient between the two groups (P 0.05). The activity of serum SOD,GSH-Px in the high dose group and the high dose group was significantly higher than that in the high dose group (P 0.05), but there was no significant difference in the body weight and liver organ coefficient between the two groups (P 0.05). The content of MDA decreased significantly (P 0.05), and the activities of ALT and AST decreased significantly (P 0.05). The results of pathological staining showed that polyphenols could restore the normal morphology of swollen hepatocytes. It can also reduce the phenomenon of intercellular bleeding and inflammatory cell infiltration, and the effect is positively correlated with the dose.
【作者单位】：黑龙江八一农垦大学食品学院;国家杂粮工程技术研究中心;青岛琅琊台集团股份有限公司;
【基金】：大庆市科技局创新项目(sjh-2013-65) 国家星火计划项目(2015GA670008) 黑龙江省应用技术研究与开发计划重大项目(GA15B301) 黑龙江八一农垦大学研究生创新科研项目(YJSCX2017-Y56;YJSCX2017-Y57)
【分类号】：R151

【相似文献】